:: Main :: About :: Current Articles :: Archive :: Article In Press :: Submit manuscript :: Contact ::
:: ::
Back to the articles list Back to browse issues page
Distribution of Genes Encoding Iron Uptake Systems among the Escherichia coli Isolates from Diarrheal Patients of Iran
Aida Hajihosein-Tabrizi , Mehri Habibi , Mohsen Tabasi , Mohammad Reza Asadi Karam
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (112 Views)
Introduction: Diarrheagenic Escherichia coli (DEC) including enteropathogenic (EPEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and shiga toxin producing E. coli are among the most common agents of diarrhea. There are various classes of iron uptake receptors, but there is not much data on the presence of these iron receptors in DEC isolates. The present study aimed to evaluate the presence of iron receptor genes and also hemolysis activity in these isolates. Methods: Totally, 88 DEC isolates (EAEC, ETEC, STEC, and EPEC) from a previous microbial collection were included in this study. The isolates were tested for the production of hemolysin on blood agar plates. Then, Polymerase Chain Reaction (PCR) was used for detection of iron acquisition genes, including chuA, hma, iroN, fyuA, iutA and ireA. Results: Our results showed that 8 (66.7%), 25 (89.3%), 17 (44.4%) and 10 (83.4%) of EPEC, STEC, ETEC and EAEC isolates, respectively had hemolytic activity. All the EPEC isolates were negative for hma gene, and iroN and ireA genes were absent in the EAEC isolates. The frequency of chuA, hma and fyuA genes in the STEC and EAEC isolates was higher, whereas EPEC and ETEC isolates revealed a higher frequency of iroN gene than the STEC and EAEC isolates. Conclusion: This study reports the presence of various iron receptor genes with a significant hemolysin activity in DEC isolates from Iran. The presence of these genes may contribute to the increased pathogenesis of these isolates in the intestinal tract.
Keywords: Diarrheagenic, Escherichia coli, Iron receptors, Hemolysin, PCR
     
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2018/05/7 | Accepted: 2018/06/2
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print



Back to the articles list Back to browse issues page
Journal of Medical Microbiology and Infectious Diseases
Persian site map - English site map - Created in 0.21 seconds with 31 queries by YEKTAWEB 3735