Volume 12, Issue 3 (9-2024)                   JoMMID 2024, 12(3): 201-207 | Back to browse issues page

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Moradi Pordanjani P, Bolhassani A, Agi E. Differential Immunostimulatory Effects of EGFP and +36 GFP on Immune Cells. JoMMID 2024; 12 (3) :201-207
URL: http://jommid.pasteur.ac.ir/article-1-698-en.html
Differential Immunostimulatory Effects of EGFP and +36 GFP on Immune Cells
Parisa Moradi Pordanjani , Azam Bolhassani * , Elnaz Agi
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (102 Views)
Introduction: Green fluorescent protein (GFP) and its variants are pivotal in tracking gene expression across various gene delivery systems. While GFP is typically employed for intracellular reporting, it can be modified to display on cell surfaces for labeling. Previous research indicates GFP might have immunogenic effects, notably enhancing tumor-specific T cell responses. This study explores the immunostimulatory differences between enhanced GFP (EGFP) and the supercharged variant, +36 GFP. Methods: Recombinant EGFP and +36 GFP proteins were generated using an Escherichia coli expression system. Murine bone marrow-derived dendritic cells (BMDCs) were generated using established protocols. Splenocytes were isolated from murine spleens via mechanical disruption and red blood cell lysis. The RAW 264.7 macrophage cell line was cultured in complete DMEM medium. Immune cells were then incubated with varying concentrations of EGFP and +36 GFP, separately, for 48 h. Cytokine levels (IFN-γ, TNF-α, IL-10) were quantified using sandwich ELISA.
Keywords: Green Fluorescent Protein, Enhanced GFP, Supercharged +36 GFP, Immunogenicity, Cytokines
Full-Text [PDF 1033 kb]   (62 Downloads)    
Type of Study: Original article | Subject: Immune responses, deficiencies and vaccine candidates
Received: 2024/12/9 | Accepted: 2024/09/11 | Published: 2024/12/22
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.