Volume 12, Issue 2 (6-2024)                   JoMMID 2024, 12(2): 138-149 | Back to browse issues page

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Malik A S, Chishti M S, Bashir G, Ahangar I N. Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital. JoMMID 2024; 12 (2) :138-149
URL: http://jommid.pasteur.ac.ir/article-1-630-en.html
Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital
Amir Sayed Malik , Mohammed Sameer Chishti , Gulnaz Bashir * , Irfan Nisar Ahangar
Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Union Territory of Jammu and Kashmir, Pin 190011, India
Abstract:   (269 Views)
Introduction: Uropathogenic Escherichia coli (UPEC) is a leading cause of community-acquired and healthcare-associated infections, and antimicrobial resistance in UPEC poses significant challenges to managing these infections. This study aimed to investigate the molecular types of UPEC using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and analyze their resistance patterns in a tertiary care setting. Methods: A cross-sectional study was conducted at a tertiary care hospital, where 65 consecutive E. coli isolates from urinary specimens were collected. Isolates were identified biochemically and confirmed by 16S rRNA gene PCR. Antibiotic susceptibility testing was conducted following CLSI guidelines, and molecular typing was performed using ERIC-PCR. ERIC-PCR profiles were analyzed using PAST software version 4.0, generating a dendrogram to visualize similarity among ERIC types. Fisher's exact test was used to determine if specific ERIC types were significantly associated with particular antibiotic resistance profiles. Results: The results showed that 95% of the isolates were resistant to at least two antibiotics, with 92.3% being multidrug resistant (MDR). The highest resistance was observed against ampicillin, while no resistance was seen against colistin and tigecycline. The resistant isolates displayed 36 different antibiograms, indicating a significant degree of resistance variability. ERIC-PCR typing revealed 22 unique clusters at a similarity coefficient of approximately 70%, highlighting the genetic diversity of UPEC isolates in our setting. Conclusion: This study enhances the understanding of UPEC epidemiology in healthcare by revealing the molecular characteristics and resistance profiles of prevalent strains. The high occurrence of MDR UPEC and the absence of a correlation between ERIC types and antibiograms suggest adaptability and increased resistance. These results highlight the necessity for continuous surveillance to inform infection control measures and direct targeted interventions against the spread of MDR UPEC.
 
Keywords: UPEC, Enterobacterial repetitive intergenic consensus, PCR, MDR, ERIC-PCR typing, 16s rRNA gene, Disc diffusion method, Antibiotypes
Full-Text [PDF 884 kb]   (61 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2023/12/6 | Accepted: 2024/09/20 | Published: 2024/09/18
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.