Volume 11, Issue 4 (12-2023)                   JoMMID 2023, 11(4): 200-212 | Back to browse issues page

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Muhović B, Islam N, M. Tambuwala M, Hromić-Jahjefendić A. Characterizing Lactic Acid Bacteria and Thermotolerant Coliforms in Spoiled Dairy Products from Bosnia and Herzegovina. JoMMID 2023; 11 (4) :200-212
URL: http://jommid.pasteur.ac.ir/article-1-592-en.html
Characterizing Lactic Acid Bacteria and Thermotolerant Coliforms in Spoiled Dairy Products from Bosnia and Herzegovina
Berina Muhović , Nadia Islam , Murtaza M. Tambuwala , Altijana Hromić-Jahjefendić *
Department of Genetics and Bioengineering, International University of Sarajevo, Bosnia and Herzegovina
Abstract:   (953 Views)
Introduction: The rising foodborne disease outbreaks poses significant challenges to key objectives in food microbiology. This trend is primarily attributed to global population growth and intensified food production. A thorough microbiological assessment of end products is therefore crucial. Methods: We evaluated the bacterial presence and abundance in various dairy products (sour cream, cottage cheese, buttercream, cream cheese, pasteurized milk, protein-rich milk, and yogurt) sourced from a local supermarket in Bosnia and Herzegovina. Two enumeration methods (pour plating and most probable number) were employed alongside morphological, biochemical, and molecular analyses (Gram staining, oxidase test, catalase test, indole test, lipolytic activity assay, and RT-qPCR). Our focus was on spoilage-causing lactic acid bacteria (LAB), hygiene indicator thermotolerant coliforms (TC), and the foodborne pathogen Salmonella spp.  Results: Six out of seven dairy products harbored high levels of LAB, suggesting potential spoilage, except for cottage cheese. Additionally, both TC and Escherichia coli exceeded acceptable microbial limits, particularly in pasteurized milk. Furthermore, initial tests detected presumptive Salmonella spp. in cream cheese, protein-rich milk, and yogurt. Conclusion: These results highlight the need for stringent sanitary practices during dairy production to extend product shelf-life and prevent premature spoilage from unwanted bacterial presence. Moreover, eliminating pathogen contamination during manufacturing is crucial to mitigate serious food safety risks, including potential food poisoning.

 
Keywords: Lactic Acid Bacteria, Thermotolerant Coliforms, Salmonella spp., Dairy products, Spoilage, Shelf-life extension, Dairy processing, Bosnia and Herzegovina
Full-Text [PDF 1120 kb]   (313 Downloads)    
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2023/08/30 | Accepted: 2023/12/10 | Published: 2024/02/24
References
1. Coutts AM. Review and discussion of the body's normal microorganisms. Br J Nurs. 2006; 15 (16): 864-8. [DOI:10.12968/bjon.2006.15.16.21850] [PMID]
2. G Greub, C Holliger, D Sanglard, J Schrenzel, V Thiel, P Viollier. The Swiss Society of Microbiology: Small Bugs, Big Questions and Cool Answers. CHIMIA. 2016; 70 (12): 874-7. [DOI:10.2533/chimia.2016.874] [PMID]
3. E Ruiz de Gopegui Bordes, M del Remedio Guna Serrano, N Orta Mira, M Rosario Ovies, M Poveda, C. Gimeno Cardona, Análisis de resultados del Programa Externo de Control de Calidad SEIMC. Año 2010. Enfermedades Infecc Microbiol Clínica. 2011; 29 (5): 1-7. [DOI:10.1016/S0213-005X(11)70037-4] [PMID]
4. J Wang, RA Barke, J Ma, R Charboneau, S Roy, Opiate abuse, innate immunity, and bacterial infectious diseases. Arch Immunol Ther Exp. (Warsz.). 2008; 56 (5): 299-309. [DOI:10.1007/s00005-008-0035-0] [PMID]
5. Bintsis T. Microbial pollution and food safety. AIMS Microbiol. 2018; 4 (3): 377-396. [DOI:10.3934/microbiol.2018.3.377] [PMID] []
6. S Brul. Preservative agents in foods Mode of action and microbial resistance mechanisms. Int J Food Microbiol. 1999; 50 (1-2): 1-17. [DOI:10.1016/S0168-1605(99)00072-0] [PMID]
7. Chandan RC. Enhancing Market Value of Milk by Adding Cultures. J Dairy Sci. 1999; 82 (10): 2245-56. [DOI:10.3168/jds.S0022-0302(99)75472-X] [PMID]
8. NH Martin, A Trmčić, T-H Hsieh, KJ Boor, M Wiedmann. The Evolving Role of Coliforms As Indicators of Unhygienic Processing Conditions in Dairy Foods. Front Microbiol. 2016; 7: 1549. [DOI:10.3389/fmicb.2016.01549] [PMID] []
9. TM Cogan, TP Beresford, J Steele, J Broadbent, NP Shah, Z Ustunol. Invited Review: Advances in Starter Cultures and Cultured Foods. J Dairy Sci. 2007; 90 (9): 4005-21. [DOI:10.3168/jds.2006-765] [PMID]
10. P. D. Cotter, C. Hill, and R. P. Ross, Bacteriocins: developing innate immunity for food. Nat Rev Microbiol. 2005, vol. 3, no. 10, pp. 777-788. [DOI:10.1038/nrmicro1273] [PMID]
11. R Souad, HT Mossadak, B Leila. Assessing hygiene indicators in two dairies in Algeria in producing pasteurized milk. Vet World. 2021; 14 (9): 2317-24. [DOI:10.14202/vetworld.2021.2317-2324] [PMID] []
12. MG Wacker, C Janas, FS Ferreira, FP Vieira. Manufacturing and Safety Guidelines for Manufactured Functionalized Nanomaterials in Pharmaceutics. Biomed Appl Functional Nanomater. 2018; 623-51. [DOI:10.1016/B978-0-323-50878-0.00021-5]
13. CL Baylis, S MacPhee, R P Betts. Comparison of two commercial preparations of buffered peptone water for the recovery and growth of Salmonella bacteria from foods: comparison of bpw for Salmonella. J Appl Microbiol. 2000; 89 (3): 501-10. [DOI:10.1046/j.1365-2672.2000.01145.x] [PMID]
14. The science of food security | npj Science of Food. Accessed: Feb. 18, 2023. [Online]. Available: https://www.nature.com/articles/s41538-018-0021-9
15. D Wessels, PJ Jooste, JF Mostert. Psychrotrophic, proteolytic and lipolytic properties of Enterobacteriaceae isolated from milk and dairy products. Int J Food Microbiol. 1989; 9 (1): 79-83. [DOI:10.1016/0168-1605(89)90040-8] [PMID]
16. The shelf-life of dairy products: 1. Factors influencing raw milk and fresh products. Accessed: Feb. 18, 2023. [Online]. Available: https://www.researchgate.net/publication/229562776_The_shelf-life_of_dairy_products_1_Factors_influencing_raw_milk_and_fresh_products
17. CT Butterfield. Comparison of the Enumeration of Bacteria by Means of Solid and Liquid Media. Public Health Rep. 1933; 48 (42): 1292. [DOI:10.2307/4580955]
18. Davis C. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. J Microbiol Methods. 2014; 103: 9-17. [DOI:10.1016/j.mimet.2014.04.012] [PMID]
19. H Naghili, H Tajik, K Mardani, SM Razavi Rouhani, A Ehsani, P Zare. Validation of drop plate technique for bacterial enumeration by parametric and nonparametric tests. Vet Res Forum. 2013; 4 (3): 179-83.
20. RB Moyes, J Reynolds, DP Breakwell. Differential Staining of Bacteria: Gram Stain. Curr Protoc Microbiol. 2009; 15 (1). [DOI:10.1002/9780471729259.mca03cs15] [PMID]
21. Estimating probability distributions of bacterial concentrations in food based on data generated using the most probable number (MPN) method for use in risk assessment | Request PDF. Accessed: Feb. 19, 2023. [Online]. Available: https://www.researchgate.net/publication/257398792_Estimating_probability_distributions_of_bacterial_concentrations_in_food_based_on_data_generated_using_the_most_probable_number_MPN_method_for_use_in_risk_assessment
22. Catalase production and sensitiveness to hydrogen peroxide amongst bacteria: With a scheme of classification based on these properties. Accessed: Feb. 18, 2023. [Online]. Available: https://www.researchgate.net/publication/229753940_Catalase_production_and_sensitiveness_to_hydrogen_peroxide_amongst_bacteria_With_a_scheme_of_classification_based_on_these_properties
23. Biochemical tests for identification of medical bacteria - Ghent University Library. Accessed: Feb. 18, 2023. [Online]. Available: https://lib.ugent.be/en/catalog/rug01:000694128
24. B Remenant, E Jaffrès, X Dousset, M-F Pilet, M Zagorec. Bacterial spoilers of food: Behavior, fitness and functional properties. Food Microbiol. 2015; 45 (Pt A): 45-53. [DOI:10.1016/j.fm.2014.03.009] [PMID]
25. S. Roller, Physiology of food spoilage organisms, Int J Food Microbiol. 1999, vol. 50, no. 1-2, pp. 151-153. [DOI:10.1016/S0168-1605(99)00083-5] [PMID]
26. EG Dudley. Food Microbiology: Fundamentals and Frontiers, 5th Edition. Emerg Infect Dis. 2022; 28 (1): 267. [DOI:10.3201/eid2801.211862] []
27. A Rompré, P Servais, J Baudart, M-R de-Roubin, P Laurent. Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. J Microbiol Methods. 2002; 49 (1): 31-54. [DOI:10.1016/S0167-7012(01)00351-7] [PMID]
28. L Ramnath, B Sithole, R Govinden. Identification of lipolytic enzymes isolated from bacteria indigenous to Eucalyptus wood species for application in the pulping industry. Biotechnol Rep. 2017; 15: 114-124. [DOI:10.1016/j.btre.2017.07.004] [PMID] []
29. A Viet-Phuong Le, D Huang, T Blick, EW Thompson, A Dobrovic. An optimised direct lysis method for gene expression studies on low cell numbers. Sci Rep. 2015; 5 (1): 12859. [DOI:10.1038/srep12859] [PMID] []
30. Craig N, Fletcher SL, Daniels A, Vewman C, O'Shea M, Tan WS, et al. Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification. Viruses. 2022; 14 (3): 508. [DOI:10.3390/v14030508] [PMID] []
31. JCF Pantoja, DJ Reinemann, PL Ruegg. Factors associated with coliform count in unpasteurized bulk milk. J Dairy Sci. 2011; 94 (6): 2680-91. [DOI:10.3168/jds.2010-3721] [PMID]
32. Pacheco DO, Bairros JV, Passos LDF, Buchweitz MRD, Rodrigues KL, Helbig E, et al. Total and Thermo-Tolerant Coliforms, Salmonella spp., Escherichia coli, Pseudomonas spp and Listeria monocytogenes in Broilers Chicken Meat Processing Chain in Southern Brazil. J Food Nutr Res. 2017; 5 (11): 867-73. [DOI:10.12691/jfnr-5-11-11]
33. Principles of Modern Microbiology: 9780763710750." Accessed: Feb. 20, 2023. [Online]. Available: https://www.jblearning.com/catalog/productdetails/9780763710750
34. H Yue, B Zhang, X Zhu, H Zhang, C Tang. Comparison of Culture Methods for Isolation of Salmonella in Yak Fecal Samples. Indian J Microbiol. 2014; 54 (2): 223-6. [DOI:10.1007/s12088-013-0423-y] [PMID] []
35. B Zhang, Y Wang, Z Tan, Z Li, Z Jiao, Q Huang. Screening of Probiotic Activities of Lactobacilli Strains Isolated from Traditional Tibetan Qula, A Raw Yak Milk Cheese. Asian-Australas J Anim Sci. 2016; 29 (10): 1490-9. [DOI:10.5713/ajas.15.0849] [PMID] []
36. J Park, J Kim, K Kim. Acid resistance and acid adaptation responses of foodborne Escherichia coli isolates. J Food Saf. 2017; 37 (4): e12352. [DOI:10.1111/jfs.12352]
37. Muller A, Schader C, Scialabba NE, Brüggemann J, Isensee A, Erb K, et al. Strategies for feeding the world more sustainably with organic agriculture. Nat Commun. 2017; 8 (1): 1290. [DOI:10.1038/s41467-017-01410-w] [PMID] []
38. Environmental impact of food equity | Nature Human Behaviour. Accessed: Feb. 20, 2023. [Online]. Available: https://www.nature.com/articles/s41562-019-0773-5
39. R. J. Blodgett, Measuring improbability of outcomes from a serial dilution test. Commun Stat. - Theory Methods. 2002; 31 (12): 2209-23. [DOI:10.1081/STA-120017222]
40. JP Audia, CC Webb, JW Foster. Breaking through the acid barrier: An orchestrated response to proton stress by enteric bacteria. Int J Med Microbiol. 2001; 291; (2): 97-106. [DOI:10.1078/1438-4221-00106] [PMID]
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.