Volume 2, Issue 2 (4-2014)                   JoMMID 2014, 2(2): 84-90 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khorvash F, Yazdani M R, Shabani S, Alizadeh H, Soudi A A, Shoaei P, et al . Detection of Different Types of Metallo-β-Lactamases among Pseudomonas aeruginosa Isolates Obtained from Intensive Care Unit Patients. JoMMID. 2014; 2 (2) :84-90
URL: http://jommid.pasteur.ac.ir/article-1-58-en.html
Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract:   (6360 Views)

Introduction: Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to various antimicrobial agents, such as Metallo-β-Lactams (MBL). This study aimed to investigate the prevalence of β-lactamases encoding genes among P. aeruginosa strains isolated from intensive care unit (ICU) patients by phenotypic and multiplex PCR methods. Methods: A total of 48 non-duplicate strains of P. aeruginosa were collected from different clinical specimens of patients hospitalized in ICU wards of a teaching hospital in Isfahan, Iran. Susceptibility test was performed by disk diffusion method. All meropenem resistant strains were subjected to modified Hodge test (MHT) for detection of carbapenemases. Multiplex PCRs were performed to detect β-lactam-resistant P. aeruginosa isolates. Results: In disk diffusion method, P. aeruginosa strains showed the most (97.9%) resistance against imipenem and meropenem and the least (45.8%) against colistin. Thirty-six (75%) out of the 48 isolates were multidrug resistant. PCR amplification of β-lactamase genes showed the presence of blaVIM genes in 7 (14.6%) and blaIMP in 15 (31.3%) strains. Also, blaSME, SPM, GIM, AIM and NDM genes were not observed in any of the strains. We only found a statistically significance difference between the presence of blaIMP gene and multidrug-resistant (MDR) positivity and source of specimen (p=0.009 and 0.002, respectively). Conclusion: Rapid and reliable identification of MBLs appears to be necessary for effective treatment of related infections. Besides, our results may provide useful perception to make a more appropriate choice of antibiotics, which may put a stop to carbapenem-resistant infections.

Full-Text [PDF 649 kb]   (2263 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2015/03/14 | Accepted: 2015/05/20 | Published: 2015/08/16

Add your comments about this article : Your username or Email:

Send email to the article author

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.