Volume 11, Issue 3 (9-2023)                   JoMMID 2023, 11(3): 136-140 | Back to browse issues page

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Setayesh Nia M, Elikaei A, Abdi Ali A, Rezaei Z. Isolation and Identification of a Sewage Pseudomonas aeruginosa-infecting Bacteriophage. JoMMID 2023; 11 (3) :136-140
URL: http://jommid.pasteur.ac.ir/article-1-567-en.html
Isolation and Identification of a Sewage Pseudomonas aeruginosa-infecting Bacteriophage
Mahshid Setayesh Nia , Ameneh Elikaei * , Ahya Abdi Ali , Zeynab Rezaei
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract:   (1149 Views)
Introduction: Pseudomonas aeruginosa, a nosocomial pathogen, causes respiratory infections, particularly in individuals with cystic fibrosis (CF); it poses a significant challenge due to escalating antibiotic resistance. Phage therapy is emerging as a potential alternative approach to treating antibiotic-resistant bacteria. This study aimed to isolate and identify a highly efficacious bacteriophage targeting P. aeruginosa. Methods: Sewage samples from Mofid Children's Hospital were collected and titrated using a double-layer plaque assay. After plaque detection, the phage was isolated, and then the host range, adsorption rate, and stability were evaluated through spot tests and double-layer plaque assays. Phage morphology was determined using transmission electron microscopy (TEM). Results: Of the three sewage samples collected, one contained phage. We isolated a lytic bacteriophage that exhibited clear plaque formation and induced lysis in planktonic P. aeruginosa. TEM analysis revealed that the phage belonged to the Myoviridae family. The phage had a broad host range and demonstrated optimal stability at 37 °C and pH 7. Conclusion: The isolated phage can potentially be used for treating P. aeruginosa lung infections, offering an alternative approach to address drug-resistant isolates.
 
Keywords: Bacteriophage, Cystic fibrosis, Myoviridae, Phage therapy, Pseudomonas aeruginosa, Antibiotic resistance
Full-Text [PDF 1024 kb]   (376 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2023/06/17 | Accepted: 2023/09/10 | Published: 2023/11/11
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.