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Jahantigh M, Seyedzayi N. Dominant EAEC and Widespread Antibiotic Resistance among Diarrheagenic Escherichia coli in Children with Acute Diarrhea in Zahedan, Iran. JoMMID 2024; 12 (1) :59-66
URL: http://jommid.pasteur.ac.ir/article-1-545-en.html
Dominant EAEC and Widespread Antibiotic Resistance among Diarrheagenic Escherichia coli in Children with Acute Diarrhea in Zahedan, Iran
Mojdeh Jahantigh * , Nasrin Seyedzayi
Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran
Abstract:   (717 Views)
Introduction: Diarrheal disease is a major cause of morbidity and mortality among children under the age of 10 worldwide, particularly in low- and middle-income countries. Diarrheagenic Escherichia coli (DEC) is a common cause of gastroenteritis in children. This study investigated the frequency, virulence markers, and antibiotic resistance patterns of DEC in children below 10 years with acute diarrhea in Zahedan, Iran. Methods: In this cross-sectional study, 300 E. coli isolates were collected from stool samples of children aged below 10 years with diarrhea who presented to hospitals and clinical laboratories in Zahedan. DEC pathotypes were identified using multiplex PCR and confirmed by standard biochemical tests and polyvalent antisera. Results: Of the 300 E. coli isolates examined, 89 (29.6%) were identified as diarrheagenic E. coli (DEC) using polyvalent antisera targeting known DEC pathotypes. Enteroaggregative E. coli (EAEC) was identified in 31 isolates (34.83%) based on reaction with antiserum No. 1. Enterotoxigenic E. coli (ETEC) was identified in 35 isolates (39.33%) based on reaction with antiserum No. 2. Enteropathogenic E. coli (EPEC) was identified in 23 isolates (25.84%) based on reaction with antiserum No. 3 (anti-coli3). Multiplex PCR identified the most common pathotype as EAEC (37.6%), followed by EPEC (21.7%), ETEC (15.9%), and EIEC (11.5%). Statistical analysis revealed no significant correlation between the presence of specific virulence genes (e.g., eae, pcvd432, elt, est, and iaH) and antibiotic resistance patterns in the DEC isolates. Conclusion: Given the distribution of DEC pathotypes among children in Zahedan and their increased antibiotic resistance, antibiotic treatment should be guided by molecular typing and antimicrobial susceptibility testing of isolates, when appropriate.
Keywords: Diarrheagenic E. coli (DEC), Antibiotic resistance, Enteroaggregative E. coli (EAEC), Children, Zahedan, Multiplex PCR
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Type of Study: Original article | Subject: Epidemiologic studies including microbial genotyping, phenotyping and serotyping
Received: 2023/05/8 | Accepted: 2024/05/21 | Published: 2024/06/8
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.