Volume 11, Issue 3 (9-2023)                   JoMMID 2023, 11(3): 148-154 | Back to browse issues page

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Shrestha R, Ghaju P, Kumar Chaudhary D, Kumar Karn R, Kumar Thakur R, Jaiswal S et al . Correlation between Biofilm Formation and Multi-Drug Resistance among Clinical Isolates. JoMMID 2023; 11 (3) :148-154
URL: http://jommid.pasteur.ac.ir/article-1-493-en.html
Correlation between Biofilm Formation and Multi-Drug Resistance among Clinical Isolates
Rubina Shrestha , Pooja Ghaju , Dilip Kumar Chaudhary , Raushan Kumar Karn , Rajesh Kumar Thakur , Suresh Jaiswal , Ram Krishna Shrestha *
Department of Microbiology, Modern Technical College, Pokhara University, Kathmandu, Nepal
Abstract:   (1067 Views)
Introduction: Biofilms are often found in communities of microorganisms in chronic and persistent infections, exhibiting high resistance against antimicrobial agents. Biofilm serves as a barrier, impeding the penetration of drugs and constraining their effectiveness. Multiple methods, such as the Tissue Culture Plate method, Congo Red Agar method, Tube method, bioluminescent assay, and fluorescent microscopic examination, can be used to evaluate biofilm production. Methods: The study included a total of 300 clinical isolates representing a range of bacterial species, including Acinetobacter baumannii (n=9), Coagulase Negative Staphylococcus (n=7), Enterobacter aerogenes (n=7), Enterococcus faecalis (n=15), Escherichia coli (n=137), Klebsiella pneumoniae (n=23), Proteus mirabilis (n=4), Pseudomonas aeruginosa (n=16), Salmonella typhi (n=11), and Staphylococcus aureus (n=68). Associations among isolates capable and incapable of biofilm formation and their multidrug resistance phenotypes were evaluated. Results: Among the 300 clinical isolates tested, 289 isolates (96.3%) exhibited biofilm formation. The most prevalent biofilm-forming organisms were A. baumannii (n=9), Citrobacter koseri (n=1), Coagulase Negative Staphylococcus (CONS) (n=7), E. aerogenes (n=7), E. faecalis (n=15), E. coli (n=137), Klebsiella oxytoca (n=1), K. pneumoniae (n=23), P. mirabilis (n=4), P. aeruginosa (n=16), S. typhi (n=11), S. aureus (n=68), and Streptococcus pneumoniae (n=1). The biofilm-forming isolates demonstrated increased resistance compared to isolates that did not form biofilms. Conclusion: Antimicrobial resistance represents a critical characteristic of infections involving biofilms. The study identified biofilm production in 92.7% of the isolates tested via TCP and in 72.3% of the isolates using the CRA. Furthermore, it was observed that pathogens with multidrug resistance (MDR) exhibited a higher biofilm production tendency than non-producing pathogens.
 
Keywords: Biofilm, Resistance, Pathogens, Chronic infection, Multidrug resistance, Antimicrobial agents
Full-Text [PDF 632 kb]   (377 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2022/07/29 | Accepted: 2023/09/10 | Published: 2023/11/11
References
1. Li chen, Yu-mei Wen. The role of bacterial biofilm in persistent infections and control strategies. Int J Oral Sci. 2011; 3 (2): 66-73.
2. Jamal M, Tasneem U, Hussain T, Andleeb S. Bacterial Biofilm: Its Composition. Formation and Role in Human Infections. J Microbio Biotechnol. 2015; 4 (3): 1-14.
3. Oli AK, Javaregowda PK, Jain A, Kelmani CR. Mechanism Involved in Biofilm Formation of Enterococcus Faecalis. 2022.
4. Fux C, et al. Survival strategies of infectious biofilms. Trends Microbiol. 2005; 13 (1): 34-40.
5. Patricia M. tille, PhD, MLS (ASCP): Bailey & Scott’s Diagnostic Microbiology: 13th Edition: 35.
6. Tayal R, Baveja S, De A. Analysis of biofilm formation and antibiotic susceptibility pattern of uropathogens in patients admitted in a tertiary care hospital in India. Internat J Health Allied Sci. 2015; 4 (4): 247-49
7. Tabasi M, Eybpoosh S, Siadat SD, Elyasinia F, Soroush A, Bouzari S. Modulation of the gut microbiota and serum biomarkers after laparoscopic sleeve gastrectomy: a 1-year follow-up study. Obes Surg. 2021; 31 (5): 1949-56.
8. Thomas D, Day F. Biofilm formation by plant associated bacteria. Ann Rev Microbiol 2007; 61: 401-22
9. Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet. 2001; 358 (9276): 135-8.
10. Dardi Charan Kaur, Wankhede SV. A study of biofilm formation and Metallo-beta lactamases in Pseudomonas aeruginosa in a tertiary care rural hospital. Int J Sci Res Pu. 2013; 3 (10): 1-7.
11. Swarna SR, Supraja, Gomathi S, Radha Madhavan. A study of biofilm formation in multidrug resistant organisms. J Pharma Res. 2014; 8 (5): 660-4.
12. Hatt JK, Rather PN. Role of bacterial biofilms in urinary tract infections. Curr Top Microbiol Immunol. 2008; 322: 163-192
13. Jessica LL, Alexander RH. Staphylococus aureus biofilms: Recent developments in biofilm dispersal. Front Cell Infect Microbiol. 2014; 4: 178.
14. Parsek MR, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003; 57: 677-701.
15. Donlan MR. Biofilm Formation: A Clinically Relevant Microbiological Process. Clin Infect Dis. 2001; 33 (8): 1387-92.
16. Luke F. chen, Matthew E Falagas, Anton Y Peleg. Facing the rising tide of multidrug resistant Gram-negative pathogens. Healthcare Infect. 2011; 16: 1- 5.
17. Pradeep Kumar SS, Easwer HV, Maya Nandkumar A. Multiple drug resistant bacterial biofilms on implanted catheters – A reservoir of infection. J Assoc Phys India. 2013; 61 (10): 702-7.
18. Abdel-Aziz SM, Aeron A. Bacterial biofilm: dispersal and inhibition strategies. SAJ Biotechnol. 2014; 1 (1): 105.
19. National Audit Office (2004). Improving Patient Care by Reducing the Risk of Hospital Acquired Infection: a Progress Report. London: Stationery Office.
20. Smith K, Hunter SI. Efficacy of common hospital biocides with biofilms of multi- drug resistant clinical isolates. J Med Microbiol. 2008; 57 (Pt 8): 966-73.
21. Zolfaghan PS, Wyncoll DL. The tracheal tube: gateway to ventilator-associated pneumoniae. Crit Care. 2011; 15 (5): 310.
22. Christensen GD, Simpson WA, Younger JA, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol. 1995; 22 (6): 996-1006.
23. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun1982; 37 (1): 318-26.
24. Freeman J, Falkiner FR, Keane CT. New method for detecting lime production by coagulase negative staphylococci. J Clin Pathol. 1989; 42 (8): 872-4.
25. Donlan RM, Murga R, Bell M, Toscano CM, Carr JH, Novicki TJ, et al. Protocol for detection of biofilms on needleless connectors attached to central venous catheters. J Clin Microbiol. 2001; 39 (2): 750-3.
26. Aparna MS, Yadav S. Biofilms: microbes and disease. Braz J Infect Dis. 2008; 12 (6): 526- 30.
27. Zufferey J, Rime B, Francioli P, Bille J. Simple method for rapid diagnosis of catheter associated infection by direct Acridine orange staining of catheter tips. J Clin Microbiol. 1988; 26 (2): 175-7.
28. Stepanovic S, Vukovi D, Hola V, Di Bonaventura G, Djukic S, Cirkovix I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. APMIS. 2007; 115 (8): 891-9.
29. Costerton J., et al.; Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284 (5418): 1318-22.
30. Khan S, et al.; Isolation of Shigella species and their resistance patterns to a panel of fifteen antibiotics in mid and far western region of Nepal. Asian Pacific J Tropical Dis. 2014; 4: 30-34.
31. Verma S, Patil SS. In Vitro Biofilm Formation by Uropathogenic Bacteria and their Antibiotic Susceptibility Pattern. JKIMSU. 2016; 5 (3): 2231-4261.
32. Sanchez C, Mende K, Beckius M, Akers K, Romano D, Wenke J, et al. Biofilm formation by clinical isolates and the implications in chronic infections; BMC infect Dis. 2013; 13: 47.
33. Hassan A., Usman J., Kaleem F., Omair M., Khalid A., Iqbal M.; Evaluation of different detection methods of biofilm formation in the clinical isolates; Department of Microbiology, National University of Sciences and Technology(NUST), Islamabad, Army Medical College, Rawalpindi, Pakistan; 2011; 305-311.
34. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol. 2006; 24 (1): 25-9.
35. Saha S, Mamta DK, Damrolien S, Devi S, Krossnunpuii, Sharma KT. Biofilm production and its correlation with antibiotic resistance pattern among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital in north-east India. Int J Adv Med. 2018; 5 (4): 964- 8.
36. Summaiya AM, Urmi NJ. Assessment of Biofilm Formation by the Causative Organisms of Ventilator Associated Pneumonia at Intensive Care Unit of Tertiary Care Hospital. Nat J Med Res. 2012: 2 (1): 15-8.
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.