Volume 2, Issue 1 (1-2014)                   JoMMID 2014, 2(1): 40-44 | Back to browse issues page

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Talebzadeh M, Mohabati R, Babaie J, Amiri S, Allahyari M, Golkar M. Production of MAG1 Antigen of Toxoplasma gondii in Escherichia coli. JoMMID. 2014; 2 (1) :40-44
URL: http://jommid.pasteur.ac.ir/article-1-48-en.html
Department of Parasitology, Molecular Parasitology Laboratory, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (5323 Views)

  Introduction : Toxoplasmosis is a parasitic infection caused by the protozoan Toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. Toxoplasma tissue cyst matrix protein (MAG1) induces early humoral and cell-mediated immune responses. Previous studies suggested recombinant MAG1 as a promising antigen for serodiagnosis of Toxoplasma infection. A DNA fragment encoding mag1, comprising amino acids 50 to 207, was amplified from T . gondii RH strain and cloned in prokaryotic expression plasmid pET-15b(+). The cloned DNA fragment was sequenced and showed 100% similarity with the published sequences available in GenBank Database. Recombinant MAG1 was expressed in Escherichia coli, and was highly purified in a single step by immobilized metal ion affinity chromatography. In Western blot analysis, purified protein showed a much stronger reactivity with sera from patients with acute Toxoplasma infection, compared to those with chronic infection. MAG1 protein, in combination with other acute-phase markers might be useful in discriminating acute/reactivated Toxoplasma infections from chronic forms. J Med Microbiol Infec Dis, 2014, 1 (2): 5 pages.

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Type of Study: Original article |
Received: 2013/10/28 | Accepted: 2013/12/14 | Published: 2014/01/1

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