Volume 10, Issue 1 (3-2022)                   JoMMID 2022, 10(1): 19-23 | Back to browse issues page

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Akbari M H, Ahani Azari A, Fozouni L. Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran. JoMMID 2022; 10 (1) :19-23
URL: http://jommid.pasteur.ac.ir/article-1-442-en.html
Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran
Mohammad Hossein Akbari , Ania Ahani Azari * , Leila Fozouni
Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran.
Abstract:   (1531 Views)
Introduction: This study aimed to determine the frequency of extended-spectrum beta-lactamases (ESBL) and different drug resistance phenotypes in Enterobacteriaceae isolated from clinical specimens in Gonbad-e Kavus, Golestan Province. Methods: 220 clinical samples of urine, blood, pus, sputum, CSF, body fluids, and ear and eye discharge were collected during six months from April to September 2021 at a referral hospital. The samples were cultured on blood and MacConkey agar and incubated overnight at 37 °C. Standard biochemical tests and the API20E enteric identification system were used to identify bacteria. The Kirby-Bauer disk diffusion method determined the antibiotic resistance pattern, and the phenotypic confirmatory test was used for detecting ESBL producers. Results: 108 Enterobacteriaceae isolates were identified from different clinical specimens out of the samples. The isolates were Escherichia coli (36.1%), Klebsiella pneumonia (25%), Enterobacter cloacae (18.5%), Citrobacter freundii (11.1%) and Proteus mirabilis (9.2%). The highest resistance and susceptibility among the isolates belonged to sulfamethoxazole-trimethoprim (68.5%) and meropenem (11.1%), respectively. The highest prevalence of multidrug-resistant (MDR) and ESBL were observed in E. coli and Proteus mirabilis isolates. Conclusions: In this study, the high frequency of MDR phenotypes in the isolates may suggest an increasing trend of antibiotic resistance in Enterobacteriaceae. This could greatly impact the management and treatment of infections caused by these drug-resistant bacteria. Therefore, infection-control measures and continuous monitoring is recommended for controlling the spread of ESBL-producing strains in different geographical areas.
Keywords: Beta-lactamases, Drug-resistant, Enterobacteriaceae, Prevalence
Full-Text [PDF 3508 kb]   (780 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2022/01/11 | Accepted: 2022/03/11 | Published: 2022/04/4
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.