Volume 10, Issue 4 (12-2022)                   JoMMID 2022, 10(4): 192-198 | Back to browse issues page


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Nouri Gharajalar S, Hassanzadeh M, Mahmoudi R, Emamverdizadeh P. Antibacterial Activity of Trachyspermum ammi Essential Oil Against Streptococcus mutans Isolated from Human Dental Plaques. JoMMID 2022; 10 (4) :192-198
URL: http://jommid.pasteur.ac.ir/article-1-419-en.html
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Abstract:   (997 Views)
Introduction: Mutans Streptococci are significant pathogens isolated from dental plaques. Due to the increasing frequency of antimicrobial resistance among pathogens causing dental caries, more studies have focused on using natural agents against them. This study aimed to evaluate the antibacterial effects of Trachyspermum ammi essential oil against Streptococcus mutans isolated from dental plaques. Methods: Twenty human dental plaque samples were collected, and eight S. mutans isolates were detected using biochemical and molecular tests. The antibiotic susceptibility patterns of the isolates were determined by the disc diffusion method. Also, T. ammi essential oil antibacterial activity was investigated using the disc diffusion method and determining minimum inhibitory concentration (MIC50) and minimum bactericidal concentration (MBC) values. Results: Phenotypic and genotypic characterization identified S. mutans in 40% of the samples. The antibiotic susceptibility assay revealed the highest resistance patterns against cefotaxime (100%), ceftriaxone (100%), and penicillin (87.5%). The T. ammi essential oil demonstrated 20 µg/ml MIC and 80µg/ml MBC against S. mutans. Conclusion: The present study revealed a potent antibacterial activity for T. ammi essential oil against S. mutans isolates. 
 
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Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2021/10/24 | Accepted: 2022/12/10 | Published: 2022/12/31

References
1. Bryan A, Shapir N, Sadowsky MJ. Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected and nonclinical Escherichia coli strains isolated from diverse human and animal sources. Appl Environ Microbiol. 2004; 70 (4): 2503-7. [DOI:10.1128/AEM.70.4.2503-2507.2004]
2. Franco E Franco TCC, Amoroso P, Marin JM, Avila FAD. Detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction. Braz Dent J. 2007; 18 (4): 329-33. [DOI:10.1590/S0103-64402007000400011]
3. Bowen W H, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011; 45: 69-86. [DOI:10.1159/000324598]
4. Senadheera D, Cvitkovitch D. G. Quorum sensing and biofilm formation by Streptococcus mutans. Adv Exp Med Biol. 2008; 631: 178-88. [DOI:10.1007/978-0-387-78885-2_12]
5. Takahashi N, Nyvad B. Caries ecology revisited: microbial dynamics and the caries process. Caries Res. 2008; 42: 409-18. [DOI:10.1159/000159604]
6. Qiu W, Zhou Y, Li Z, Huang T, Xiao Y, Cheng L, et al. Application of Antibiotics/Antimicrobial Agents on Dental Caries. BioMed Research International. 2020; 5658212. [DOI:10.1155/2020/5658212]
7. Abdulrahman Salman H, Senthilkumar R, Imran H, Selvam KP. Isolation and typing of Streptococcus mutans and Streptococcus sobrinus from Caries-active Subjects. Contemp Clin Dent. 2017; 8(4): 587- 93. [DOI:10.4103/ccd.ccd_610_17]
8. Mahmoudi R, Ehsani A, Tajik H, Pajohi Alamoti M. Evaluation of phytochemical and antibacterial properties of some medicinal plants from Iran. J Biol Act Prod Nat. 2013; 3: 310-22. [DOI:10.1080/22311866.2013.867665]
9. Boskabady MH, Alitaneh S, Alavinezhad A. Carum copticum L. A herbal medicine with various pharmacological effects. BioMed Research International. 2014; 1-11. [DOI:10.1155/2014/569087]
10. Sharifimood B, Shafaghat M, Metanat M, Saeidi S, Sepehri N. The inhibitory effect of Ajwan essential oil on bacterial growth. IJID. 2014; 2 (1): e19394. [DOI:10.17795/iji-19394]
11. Inouye SH, Takizawa T, Yamaguchi H. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrob Chemother. 2001; 47 (5): 65-573. [DOI:10.1093/jac/47.5.565]
12. Nomura R, Nakano K, Nemoto H, Fujita K, Inagaki S, Takahashi T, et al. Isolation and characterization of Streptococcus mutans in heart valve and dental plaque specimens from a patient with infective endocarditis. J Med Microbiol. 2006; 55 (8): 1135-40. [DOI:10.1099/jmm.0.46609-0]
13. Gharajalar NS. Hassanzade M. Antibacterial properties of Carum copticum essential oil against mutans Streptococci isolated from dog dental plaque. Vet Med. 2017; 62: 654-60. [DOI:10.17221/180/2016-VETMED]
14. Sayah RS, Kaneene JB, Johnson Y, Miller R. Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic and wild animal fecal samples, human septage, and surface water. Appl Environ Microbiol. 2005; 71 (3): 1394-404. [DOI:10.1128/AEM.71.3.1394-1404.2005]
15. Nouri Gharajalar S, Firuz Amandi M. Molecular detection of Antibiotic resistance determinants in Lactic acid bacteria from human dental plaques. JOMMID. 2017; 5 (3- 4): 51-5. [DOI:10.29252/JoMMID.5.3.4.51]
16. Mahmoudi R, Kosari M, Barati SH. Phytochemical and Biological properties of Ferula sharifi essential oil. J Biol Act Prod Nat. 2013; 3: 331-8. [DOI:10.1080/22311866.2013.867664]
17. Nouri Gharajalar S. Antibacterial activity of ajwain essential oil against some zoonotic bacteria. JZD. 2021; 5 (2): 26-33.
18. Goudarzi GHR, Saharkhiz MJ, Sattari M, Zomorodian K. Antibacterial Activity and Chemical Composition of Ajowan (Carum copticum Benth. & Hook) Essential Oil. J Agric Sci Technol. 2011; 13: 203-8.
19. Galvao L, Furletti VF, Bersan SM, Guilherme da Cunha M, Ruiz AL, Ernesto de Carvalho J, Sartoratto A, Lúcia Garcia Rehder V, Figueira GM, Duarte MCT, Ikegaki M, Matias de Alencar S. Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects. J Evid Based Complementary Altern Med. 2012; 751435: 1-12. [DOI:10.1155/2012/751435]
20. Majdi M, Dastan D, Maroofi H. Chemical Composition and Antimicrobial Activity of Essential Oils of Ballota nigra Subsp. kurdica From Iran. Jundishapur J Nat Pharm Prod. 2017; 12 (3): e36314. [DOI:10.5812/jjnpp.36314]
21. Saravia MS, Nelson-Filho P, Yoko Ito I, Bezerra da Silva LA, Bezerra da Silva RA, Emilson CG. Morphological differentiation between S. mutans and S. sobrinus on modified SB-20 culture medium. Microbiol Res. 2011; 166 (1): 63-7. [DOI:10.1016/j.micres.2010.01.001]
22. Psoter WJ, GE Y, Russell SL, Chen Z, Katz RV, Jean-Charles G, Li Y. PCR detection of Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dental plaque samples from Haitian adolescents. Clin Oral Investig. 2011; 15 (4): 461-9. [DOI:10.1007/s00784-010-0413-y]
23. Manzo LM, Moussa I, Ikhri K. Survey: A Comprehensive Review of Medicinal Plants used Against Gastrointestinal Disorders in Niger, West Africa. Jundishapur J Nat Pharm Prod. 2017; 12 (4): e65730.
24. Rasooli I, Owlia P. Chemoprevention by thyme oils of Aspergillus parasiticus growth and aflatoxin production. Phytochemistry. 2005; 66 (24): 2851-6. [DOI:10.1016/j.phytochem.2005.09.029]
25. Soltani Howyzeh M, Sadat Noori SA, Shariati V. Essential oil profiling of Ajowan (Trachyspermum ammi) industrial medicinal plant. Ind Crops Prod. 2018; 19: 255-9. [DOI:10.1016/j.indcrop.2018.04.022]
26. Godavari A, Manicka Moorthi N, Dhinesh Kumar T. Assessment of in vitro Antibacterial activity of Trachyspermum ammi. IJPSR. 2021; 12: 50-3.
27. Rabiey S, Hosseini H, Rezaei M. Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system. Braz J Microbiol. 2014; 1: 89-96. [DOI:10.1590/S1517-83822014000100013]
28. Ganapathi A, Roy A, Geetha RV. Antimicrobial Effects of Trachyspermum ammi on Oral Microbes. IJSER. 2017; 8: 80-5.

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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.