Volume 9, Issue 4 (12-2021)                   JoMMID 2021, 9(4): 191-195 | Back to browse issues page

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Basiri S, Khalesi B, Mousavi-Nasab S D, Ivani Z, Ghaderi M. High Frequency of Salivirus in Pediatric Patients with Acute Gastroenteritis. JoMMID 2021; 9 (4) :191-195
URL: http://jommid.pasteur.ac.ir/article-1-395-en.html
High Frequency of Salivirus in Pediatric Patients with Acute Gastroenteritis
Saber Basiri , Bahman Khalesi , Seyed Dawood Mousavi-Nasab , Zahra Ivani , Mostafa Ghaderi *
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (1779 Views)
Introduction: Salivirus (SalV), a possible causative agent of gastroenteritis, belongs to the genus Salivirus in Picornaviridae Family. The fecal-oral path seems to be the entry route for SalV in susceptible peoples such as children that consume polluted water. We investigated the SalV occurrence in children's stool with acute gastroenteritis using a reverse transcription-quantitative polymerase chain reaction assay (RT-qPCR). Methods: From Sep. 2018 to May 2019, 160 stool samples were collected from children with acute gastroenteritis admitted to Imam Ali General Hospital, Alborz University of Medical Sciences, Iran. After viral RNA extraction, the 5'UTR region of the SalV genome was amplified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and the viral load was defined. Results: Of the 160 stool samples, 41 (25.6%) were positive for SalV RNA. The highest SalV detection rate was in Feb. (28.2%), and the highest viral load (6.2×107 copies/g) belonged to a 1-month-old patient. Conclusion: Salivirus occurs among Iranian children with acute gastroenteritis. Our results suggest that Salivirus might contribute to acute gastroenteritis.
Keywords: Salivirus, Acute gastroenteritis, Viral load
Full-Text [PDF 961 kb]   (681 Downloads)    
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2021/09/19 | Accepted: 2021/12/10 | Published: 2021/12/28
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.