Volume 9, Issue 4 (12-2021)                   JoMMID 2021, 9(4): 178-184 | Back to browse issues page

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Saad Shamsi S, A. Elzen A, M. Ahmad K. Burkholderia cepacia Complex, an Emerging Nosocomial Pathogen at Health Care Facilities in Sebha, Libya. JoMMID 2021; 9 (4) :178-184
URL: http://jommid.pasteur.ac.ir/article-1-360-en.html
Burkholderia cepacia Complex, an Emerging Nosocomial Pathogen at Health Care Facilities in Sebha, Libya
Shamsi Saad Shamsi * , Abdelkader A. Elzen , Khadija M. Ahmad
Department of Plant Science, Microbiology Program, Research laboratory, Faculty of Science, Sebha University
Abstract:   (1779 Views)
Introduction: Burkholderia cepacie complex (Bcc) is an emerging multidrug-resistant gram-negative bacteria frequently isolated from health care facilities worldwide. The present study investigated the prevalence of Bcc in health care settings in Sebha, Libya. Methods: Two hundred swabs were initially collected. Forty-seven nosocomial Bcc isolates were identified from three medical care facilities, i.e., 40 (20%) from Sebha Medical Center, five from the Sebha Infertility Treatment Center, and two from Althanweya Clinic. The isolates were identified using a combination of biochemical tests and USP chapter <60> Microbiological Examination of Non-Sterile Products Tests for Burkholderia Cepacia Complex guidelines. A UPGMA dendrogram was used to examine the biochemical relationship of isolates. Some of the putative virulence factors contributing to the pathogenicity of the isolates were also explored. Results:  Of the 47 isolates, 29.79% were B. cepcia, 23.40% B. cenocepcia, 12.77% B. thailandensis, 8.51% B. vietnamiensis,  6.38% B. ambifaria, B. pyrrocinia, and B. stabilis each,  4.26% B. anthina, and 2.13% B. arboris. A variation in virulence factors was observed among isolates; all (100%) isolates produced siderophore, 91% had capsules, 91% produced lipase, 89% formed a biofilm, and 49% produced alkaline protease. The UPGMA dendrogram revealed that Bcc species shared substantial phenotypical identity among themselves. Conclusion: In developing countries with limited resources, diagnostic challenges in identifying Bcc species can be resolved using selective media and USP chapter <60> guidelines.
Keywords: Bcc species, Pathogenic bacteria, Virulence factors, Nosocomial infections
Full-Text [PDF 4719 kb]   (925 Downloads)    
Type of Study: Original article | Subject: Epidemiologic studies including microbial genotyping, phenotyping and serotyping
Received: 2021/05/22 | Accepted: 2021/12/10 | Published: 2021/12/28
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.