Volume 9, Issue 3 (9-2021)                   JoMMID 2021, 9(3): 142-147 | Back to browse issues page

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Hashemi A B, Nakhaei Moghaddam M, Forghanifard M M, Yousefi E. Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR. JoMMID 2021; 9 (3) :142-147
URL: http://jommid.pasteur.ac.ir/article-1-350-en.html
Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR
Aghdas Bibi Hashemi , Mahboobeh Nakhaei Moghaddam , Mohammad Mahdi Forghanifard , Ehsan Yousefi
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (1478 Views)
Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P. aeruginosa clinical isolates simultaneously by multiplex polymerase chain reaction. Methods: Eighty-five culture plates were collected from patients suspected of Pseudomonas spp infection in Ghaem Hospital and Shahid Shourideh Clinic in Mashhad from January to February 2021. After biochemical identification of P. aeruginosa isolates and the measurement of antibiotic resistance, blaOXA-10, blaOXA-48, and rpoD genes were investigated by multiplex polymerase chain reaction in the imipenem-resistant isolates. Results: Of 82 P. aeruginosa isolates, 38 (46.34%) were resistant to imipenem, with the highest percentage to carbenicillin (69.5%). All imipenem-resistant P. aeruginosa isolates were confirmed by multiplex PCR using the primers that targeted the rpoD gene. Also, in multiplex PCR, among imipenem-resistant isolates, 10 (26.3%) and 9 (23.6%) had blaOXA-10 and blaOXA-48 genes, respectively. Conclusion: In addition to molecular identification of P. aeruginosa, the present study simultaneously detected blaOXA-10 and blaOXA-48 genes by multiplex PCR. Application of this multiplex PCR, rapid identification of patients, and timely treatment can reduce the β-lactamase gene prevalence in P. aeruginosa clinical isolates.
Keywords: Pseudomonas aeruginosa, Multiplex PCR, rpoD gene, blaOXA-10 gene, blaOXA-48 gene
Full-Text [PDF 1397 kb]   (410 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2021/04/19 | Accepted: 2021/09/19 | Published: 2021/10/12
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.