Volume 9, Issue 4 (12-2021)                   JoMMID 2021, 9(4): 185-190 | Back to browse issues page

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Hariri Monfared S, Yaghoub Kazemi M, Nemati Mansoor F, Mohammadi M. Expression Analysis of MicroRNAs, miR-20a, miR-30a, miR-210, and miR-874 in Helicobacter pylori-infected patients with or without Gastric Cancer. JoMMID 2021; 9 (4) :185-190
URL: http://jommid.pasteur.ac.ir/article-1-348-en.html
Expression Analysis of MicroRNAs, miR-20a, miR-30a, miR-210, and miR-874 in Helicobacter pylori-infected patients with or without Gastric Cancer
Saba Hariri Monfared , Mina Yaghoub Kazemi , Fahimeh Nemati Mansoor , Mahnaz Mohammadi *
Department of Biology, Faculty of Biological Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
Abstract:   (1511 Views)
Introduction. Helicobacter pylori infection is one of the primary etiological factors associated with gastric carcinogenesis. This study investigated the expression of microRNAs, miR-20a, miR-30a, miR-210, and miR-874, in H. pylori-infected patients with and without gastric cancer (GC) compared to healthy subjects. Methods. Total RNA was extracted from the plasma of H. pylori-infected GC patients (n=40), H. pylori-infected patients (n=40), and healthy individuals (n=12). The expression of microRNAs was analyzed using a reverse transcription-quantitative real-time PCR (RT-qPCR) technique. Results. The miR-20a, miR-30a, miR-210, and miR-874 exhibited higher overexpression in H. pylori-infected GC patients than healthy persons (P=0.004, 0.033, 0.023, 0.024). The H. pylori-positive patients without GC also had higher miR-20a, miR-30a, and miR-210 levels than the healthy individuals (P= 0.013, 0.036, 0.032). There were no statistical differences between H. pylori-infected GC patients and H. pylori-infected patients without GC Conclusion. The microRNAs overexpression in H. pylori-infected patients with GC might be linked to H. pylori rather than GC. Therefore, these microRNAs can be helpful in H. pylori infection diagnosis rather than predicting GC. in H. pylori-infected patients.
Keywords: Gastric cancer, Helicobacter pylori, MicroRNAs, RT-qPCR
Full-Text [PDF 2869 kb]   (916 Downloads)    
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2021/04/13 | Accepted: 2021/12/10 | Published: 2021/12/28
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.