Volume 9, Issue 3 (9-2021)                   JoMMID 2021, 9(3): 148-155 | Back to browse issues page


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Akbarizare M. GC-MS Analysis and Antimicrobial Activity of an Iranian Traditional Medicinal Smoke (Anbarnasara). JoMMID. 2021; 9 (3) :148-155
URL: http://jommid.pasteur.ac.ir/article-1-346-en.html
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Abstract:   (171 Views)
Introduction: In many countries, people use animal dung smoke to treat infections. Ancient physicians Avicenna and Zakaria Razi (Zakariyyā Rāzī) recommended these compounds to treat infections. In rural areas of Iran, people used female donkey dung (Anbarnasara) smoke to treat respiratory tract and burn wound infections. This study evaluates the antibacterial and antifungal properties of Anbarnasara smoke. Methods: The smoke from burning Anbarnasara was collected in a 50%-methanol solution. Following evaporation of methanol at 50ºC, the remaining compound was dissolved in DMSO, and various concentrations (3.1-100 mg/ml) were prepared. The antimicrobial effects of various concentrations (3.1-100 mg/ml) of Anbarnasara smoke solution (ASS) were investigated, using the agar well diffusion method on 15 different microorganisms, including eight standard microorganisms and seven bacteria species from clinical specimens. Also, GC-MS analysis was performed to identify the components in ASS. Results: Antifungal activity on Candida albicans was observed at 6.2- 100 mg/ml of ASS. Among Gram-positive and Gram-negative bacteria, the most significant inhibition zones belonged to Staphylococcus epidermidis (30.5± 0.70 mm) and Proteus mirabilis (25± 0.00 mm) at 100 mg/ml. GC-MS analysis showed 16 major peak areas, and of identified components, ~50% were phenolic compounds.  Conclusion: Our results confirmed the ancient physicians' belief in the antibacterial and antifungal properties of Anbarnasara smoke.
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Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2021/04/13 | Accepted: 2021/09/19 | Published: 2021/10/12

References
1. Belete TM. Novel targets to develop new antibacterial agents and novel alternatives to antibacterial agents. Hum Microbiome J. 2019; 11 (100052): 1-10. [DOI:10.1016/j.humic.2019.01.001]
2. Frieri M, Kumar K, Boutin A. Antibiotic resistance. J Infect Public Health. 2017; 2017 (10): 369-78. [DOI:10.1016/j.jiph.2016.08.007]
3. Ahmadian-Attari MM, Amrollahi Z, Momeni PS, Khodaii Z, Bita A, Bozorgi AH. Chemical Constituents of Donkey Dung (Anbarnasara): Questioning the Recent Claims Concerning Therapeutic Effects. Int J Enteric Pathog. 2019; 7 (1): 19-22. [DOI:10.15171/ijep.2019.05]
4. Mohagheghzadeh A, Faridi P, Shams-Ardakani M, Ghasemi Y. Medicinal smokes. J Ethnopharmacol. 2006; 2006 (108): 161-84. [DOI:10.1016/j.jep.2006.09.005]
5. Arnold H, & Gulumia, M. Pharmacopoeia of traditional medicine in Venda. J Ethnopharmacol. 1984; 12: 35-74. [DOI:10.1016/0378-8741(84)90086-2]
6. Hedberg I, & Hedberg, O. Inventory of plants used in traditional medicine in Tanzania plants of the families a Canthaceae cucurbtaceae. J Ethnopharmacol. 1982; 6 (1): 29-60. [DOI:10.1016/0378-8741(82)90070-8]
7. Razi MZ. Al, Hawi: Volume 20. Tehran; Publications of the Academy of Medical Sciences. 2005; 195- 197. In Persian.
8. Khorasani MHA. Makhzan - Ul - Adviya. Iran: Deputy Minister of Research and Technology of the Ministry of Health; 1387; 363- 365. In Persian.
9. Ebne Sina HA. Canon of Medicine. Tehran; Soroush Publications1978; 165-168. In Persian.
10. Shafiee HA, Motamedi MHK, Mina M, Taheri JB, Azimi S, Joharchi K, et al. Evaluation of cytotoxic effects of Anbarnesa on fibroblast L929: Can it be used as a mouthwash? Anc Sci Life. 2014; 33 (4): 203-7. [DOI:10.4103/0257-7941.147422]
11. Shafiee HA, Moravej-Salehi E. Anbarnesa: The Past Tradition, the Future Medicine. Iran Red Crescent Med J. 2015; 17 (12): e29536. [DOI:10.5812/ircmj.29536]
12. Negut I, Grumezescu V, Grumezescu AM. Treatment Strategies for Infected Wounds. Molecules. 2018; 23 (9): 2392. [DOI:10.3390/molecules23092392]
13. Mills BB. Vaginitis: Beyond the Basics. Obstet Gynecol Clin North Am. 2017; 44 (2): 159-77. [DOI:10.1016/j.ogc.2017.02.010]
14. Ellen R. Wald. Microbiology of acute and chronic sinusitis in children. J Allergy Clin Immunol. 1992; 90 (3 Pt 2): 452-6. [DOI:10.1016/0091-6749(92)90168-2]
15. Bottone EJ. Atypical Yersinia enterocolitica: Clinical and Epidemiological Parameters. J Clin Microbiol. 1978; 7 (6): 562-7. [DOI:10.1128/jcm.7.6.562-567.1978]
16. Mohagheghzadeh A, Faridi P, Ghasemi Y. Analysis of Mount Atlas mastic smoke: A potential food preservative. Fitoterapia. 2010; 81: 577-80. [DOI:10.1016/j.fitote.2010.01.022]
17. Jahangirian H, Haron J, Ismail M. H. S, Rafiee-Mogadam R, Afsah-Hejri L, Abdollahi Y, et al. Well diffusion method for evaluation of antibacterial activity of copper phenyl hydroxamat synthesized from Canola and Palm kernel oils. Dig J Nanomater Biostructures. 2013; 8 (3): 1263-70.
18. Yang J-F, Yang C-H, Liang M-T, Gao Z-J, Wu Y-W, Chuang L-Y. Chemical Composition, Antioxidant, and Antibacterial Activity of Wood Vinegar from Litchi chinensis. Molecules. 2016; 21 (9): 1150. [DOI:10.3390/molecules21091150]
19. Singh A, Verma R, Murari A, Agrawal A. Oral candidiasis: An overview. J Oral Maxillofac Pathol. 2014; 18 (1): S81-S5. [DOI:10.4103/0973-029X.141325]
20. Bowler P. G, Duerden B. I, & Armstrong D. G. Wound Microbiology and AssociatedApproaches to Wound Management. Clin Microbiol Rev. 2001; 14 (2): 244-69. [DOI:10.1128/CMR.14.2.244-269.2001]
21. Church D, Elsayed S, Reid O, Winston B, & Lindsay R. Burn Wound Infections. Clin Microbiol Rev. 2006; 19 (2): 403-34. [DOI:10.1128/CMR.19.2.403-434.2006]
22. Tanase C, Cos S, Muntean D-L. A Critical Review of Phenolic Compounds Extracted from the Bark of Woody Vascular Plants and Their Potential Biological Activity. Molecules.2019; 24 (6): 1182. [DOI:10.3390/molecules24061182]
23. Salaün J. Cyclopropane Derivatives and their Diverse Biological Activities. Top Curr Chem. 2000; 207: 1-67. [DOI:10.1007/3-540-48255-5_1]
24. Dhiman P, Arora N, Thanikachalam PV, Monga V. Recent advances in the synthetic and medicinal perspective of quinolones: A review. Bioorg. Chem. 2019; 92: 103291. [DOI:10.1016/j.bioorg.2019.103291]
25. Anwar M.N, Shafiqur R. Fungitoxic and cytotoxic activity of a novel compound 1,2-benzenedicarboxylic acid, diisooctyl ester of Plumbago zeylanica linn. Asian J Microbiol Biotechnol Environ Sci. 2006; 8 (3): 461-4.
26. Bosman A, Combrinck S, Merwe RR-vd, Botha B, McCrindle R. Isolation of an anthelmintic compound from Leucosidea sericea. S Afr J Bot. 2004; 70 (4): 509-11. [DOI:10.1016/S0254-6299(15)30189-7]
27. Alghamdi SS, Khan MA, El-Harty EH, Ammar MH, Farooq M, Migdadi HM. Comparative phytochemical profiling of different soybean (Glycine max (L.) Merr) genotypes using GC-MS. Saudi J Biol Sci. 2018; 25 (1): 15-21. [DOI:10.1016/j.sjbs.2017.10.014]

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