Volume 9, Issue 2 (6-2021)                   JoMMID 2021, 9(2): 71-75 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Valentine N U, Ndubuisi O N, Charity N O, Ngozika F O. Prevalence and Antibiotic Susceptibility Profile of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates in Diabetes Patients with Foot Ulcers. JoMMID 2021; 9 (2) :71-75
URL: http://jommid.pasteur.ac.ir/article-1-305-en.html
Department of Biological Sciences, Spiritan University Nneochi, Abia State, Nigeria
Abstract:   (1789 Views)
Introduction: methicillin-resistant Staphylococcus aureus (MRSA) infection is common among diabetes patients with foot ulcers. This study aimed to determine MRSA isolates prevalence and antibiotic susceptibility profile in diabetic foot ulcers (DFU) patients. Methods: A total of 204 patients with diabetic foot ulcers admitted to a tertiary hospital in Abia State, Nigeria, were included in the assay. Specimens were obtained by scraping the ulcer base or the deep portion of the wound edge using a sterile curette and were promptly sent to the laboratory for culture, identification, and antibiotic susceptibility test.  Results: The MRSA prevalence in DFU patients was 22.1% (n=45). Male patients with DFU were more infected with MRSA (n= 26, 12.7%) than females (n=19, 9.3 %), but the difference was not statistically significant (P < 0.14). The age group 41-60 years had the highest prevalence (n=27, 13.2%), statistically significant (P < 0.02). Farmers had the highest prevalence of 9.8% (n=20) while the least (0.5%) was seen in housewives (n=1) with no statistical significance (P < 0.07). The antibiotics sensitivity pattern of MRSA showed 100% sensitivity to vancomycin and chloramphenicol but 100% resistance to penicillin, ceftriaxone, and oxacillin. The multidrug-resistant index was all > 0.2. Conclusions: The prevalence of MRSA in DFU patients in a tertiary hospital in Abia State was very high, with an alarming rate of drug-resistant bacteria due to the possibility of misuse and abuse of antibiotics among the populace, which requires collaborations from all stakeholders to prevent drug resistance in the study settings.
Full-Text [PDF 974 kb]   (422 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2020/10/25 | Accepted: 2021/06/20 | Published: 2021/08/29

References
1. Zubair M, Malik A, Ahmad J. Incidence, risk factors for amputation among patients with diabetic foot ulcer in a North Indian tertiary care hospital. Foot (Edinb). 2012; 22 (1): 24-30. [DOI:10.1016/j.foot.2011.09.003]
2. Lipsky BA, Berendt AR, Cornia PB, Pile JC, Peters EJ, Armstrong DG, et al. 2012 Infectious Diseases Society of America clinical practice guideline for the diagnosis and treatment of diabetic foot infections. Clin Infect Dis. 2012; 54 (12): e132-73. [DOI:10.1093/cid/cis346]
3. Vijay V. Epidemiology of diabetic foot and management of foot problems in India. Int J Low Extrem Wounds. 2010; 9 (3): 122-6. [DOI:10.1177/1534734610380026]
4. Alizargar J, Sharif M, Sharif A. Risk factors of methicillin-resistant Staphylococcus aureus colonization in diabetic outpatients, a prospective cohort study. Int J Microbiol Res. 2013; 4 (2): 147-51.
5. Lee AS, Huttner B, Harbarth S. Control of methicillin-resistant Staphylococcus aureus. Infect Dis Clin North Am. 2011; 25 (1): 155-79. [DOI:10.1016/j.idc.2010.11.002]
6. Minhas P, Perl TM, Carroll KC. Risk factors for positive admission surveillance cultures for methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci in a neurocritical care unit. Crit Care Med. 2011; 39 (10): 2322-9. [DOI:10.1097/CCM.0b013e3182227222]
7. Awadh R, Al-Anazi. Prevalence of Methicillin-Resistant Staphylococcus aureus in a teaching hospital in Riyadh, Saudi Arabia. Biomed Res. 2009; 20 (1): 7-14
8. Wang SH, Sun ZL, Guo YJ. Methicillin-resistant Staphylo-coccus aureus isolated from foot ulcers in diabetic patients in a Chinese care hospital: risk factors for infection and prev‌alence. J Med Microbiol. 2010; 59 (Pt 10):1219-24. [DOI:10.1099/jmm.0.020537-0]
9. Omuse G, Kariuki S, Revathi G. Unexpected absence of methicillin-resistant Staphylococcus aureus nasal carriage by healthcare workers in a tertiary hospital in Kenya. J Hosp Infect. 2012; 80 (1): 71-3. [DOI:10.1016/j.jhin.2011.09.009]
10. Charan J, Biswas T. How to calculate sample size for different study designs in medical research? Indian J Psychol Med. 2013; 35 (2):121-6. [DOI:10.4103/0253-7176.116232]
11. Clinical Laboratory Standard Institute. Performance standards for antimicrobial susceptibility testing; 17th informational supplement. CLSI M100-S17. CLSI, Wayne, PA. 2007.
12. Lavery LA, Fontaine JL, Bhavan K. Risk factors for methicillin-resistant Staphylococcus aureus in diabetic foot infections. Diabet Foot Ankle. 2014; 10: 5. [DOI:10.3402/dfa.v5.23575]
13. Deribe B, Woldemichael K, Nemera G. Prevalence and factors influencing diabetic foot ulcer among diabetic patients attending Arbaminch Hospital, South Ethiopia. J Diabetes Metab. 2014; 5: 1. [DOI:10.4172/2155-6156.1000322]
14. Eleftheriadou I, Tentolouris N, Argiana V. Methicillin-re-sistant Staphylococcus aureus in diabetic foot infections. Drugs. 2010; 70 (14): 1785-97. [DOI:10.2165/11538070-000000000-00000]
15. Nwakwo BO, Abdulhadi S, Magagi A, Ihesiulor G. Methicillin resistant S. aureus and their antibiotic susceptibility pattern in Kano, Nigeria. African J Clin Exp Microbiol. 2010; 11 (1): 129-36. [DOI:10.4314/ajcem.v11i1.44088]
16. Kutlu SS, Cevahir N, Akalin S, Akin F, Dirgen Caylak S, Bastemir M. Prevalence and risk factors for methicillin-resistant Staphylococcus aureus colonization in a diabetic outpatient population: a prospective cohort study. Am J Infect Control. 2012; 40 (4): 365-8. [DOI:10.1016/j.ajic.2011.05.009]
17. Fayomi OD, Oyediran EI, Adeyemo AT, Oyekale AT. Prevalence and antibiotic resistance pattern of methicillin-resistant Staphylococcus aureus among in-patients at a tertiary health facility in Ido-Ekiti, Nigeria. Int J Lab Med. 2009; 4 (2).
18. Anas B, Sohail J, Muhammad S, Ahmed M, Mansoor T. Effects of topical Vancomycin Dressing on Methicillin-Resistant Staphylococcus Aureus (MRSA) positive diabetic foot ulcers. Pak J Med Sci. 2019; 35 (4): 1099-1103. [DOI:10.12669/pjms.35.4.368]
19. Dilnessa T, Bitew A. Prevalence and antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus isolated from clinical samples at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia. BMC Infect Dis. 2016; 16 (1): 398. [DOI:10.1186/s12879-016-1742-5]
20. Shibabaw A, Abebe T, Mihret A. Antimicrobial susceptibility pattern of nasal Staphylococcus aureus among Dessie referral hospital health care workers, Dessie, Northeast Ethiopia. Int J Infect Dis. 2014; 25: 22-25. [DOI:10.1016/j.ijid.2014.03.1386]
21. Murugan S, Mani KR, Uma DP. Prevalence of Methicillin-Resistant Staphylococcus aureus among Diabetes Patients with Foot Ulcers and their Antimicrobial Susceptibility Pattern. J Clin Diagn Res. 2008; 2 (4): 979-84.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.