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Shahdadi F, Payandeh M, Salehi Sardoei A. Comparison of Antioxidant Activity of Dracocephalum polychaetum Bornm and Nepeta cataria L. and Their Effect on Probiotic Bacteria in a Simulated Gastrointestinal Environment. JoMMID 2021; 9 (1) :5-11
URL: http://jommid.pasteur.ac.ir/article-1-293-en.html
Comparison of Antioxidant Activity of Dracocephalum polychaetum Bornm and Nepeta cataria L. and Their Effect on Probiotic Bacteria in a Simulated Gastrointestinal Environment
Fatemeh Shahdadi , Maryam Payandeh , Ali Salehi Sardoei *
Ph. D. student in Horticulture Sciences Biotechnology, Faculty of Plant Production, Gorgan University of Agriculture and Natural Resources
Abstract:   (2585 Views)
Introduction: Dracocephalum polychaetum Bornm and Nepeta cataria L. are two plants from the Lamiaceae family with antibacterial, antifungal, and antiviral properties. This study evaluated the phenolic compounds, antioxidant activity, and effect of aqueous extracts on the survival of Lactobacillus acidophilus and Bifidobacterium animalis in a simulated gastrointestinal environment. Method: The aerial parts of plants were collected at the vegetative growth stage from the Hanza-Kuh's highlands in the Bahr Asman region of Jiroft city, Iran, in spring 2018. The total phenolic content of plants and antioxidant activity were measured using Folin–Ciocalteau and DPPH (2, 2-diphenyl-1-picrylhydrazyl) methods, respectively. For investigating the survival of probiotic bacteria in a simulated gastrointestinal environment, bacterial suspension was inserted into tubes containing 0, 100, 250, 500, and 1000 ppm of extracts and then incubated in a simulated gastrointestinal environment. The probiotic bacteria were counted using an MRS agar medium at various incubation times. Results: The results showed that the amount of total phenolic compounds in the D. polychaetum Bornm extract (44.55 mg/g dry matter) was higher than that of N. cataria L. (18.37 mg/g dry matter). With increased extracts concentrations, the percentage of DPPH-free radicals increased, and D. polychaetum Bornm extract in all concentrations showed higher DPPH free radical inhibitory content compared to the N. cataria L. extract. The viability results in the same gastrointestinal environment showed that samples containing N. cataria extract had a more remarkable survival rate than the controls and D. polychaetum Bornm extract. Conclusion: Using less than 500 ppm of D. polychaetum Bornm and N. cataria L. aqueous extracts can increase probiotic bacteria growth and viability.
Keywords: Dracocephalum polychaetum Bornm, Nepeta cataria L., antioxidant activity, Probiotic bacteria
Full-Text [PDF 1060 kb]   (984 Downloads)    
Type of Study: Original article | Subject: Animal Models
Received: 2020/10/20 | Accepted: 2021/03/20 | Published: 2021/04/27
References
1. Adiguzel A, Ozer H, Sokmen M, Gulluce M, Sokmen A, Kilic H, Sahin F, Baris O. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. Pol J Microbiol. 2009; 58 (1): 69-76.
2. Agboola SO, Tesic MR. Influence of Australian native herbs on the maturation of vaccum-packed cheese. LWT-Food Sci Technol. 2002; 35 (7): 575-83. [DOI:10.1016/S0023-6438(02)90917-5]
3. Amin Q. Traditional Iranian Medicines. Research publications of the Ministry of Health. Second edition. 2008.
4. Anal AK, Singh H. Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Food Sci Technol. 2007; 18 (5): 240-51. [DOI:10.1016/j.tifs.2007.01.004]
5. Anandjiwala S, Bagul MS, Parabia M, Rajani M. Evaluation of Free Radical Scavenging Activity of an Ayurvedic Formulation, Panchvalkala. Indian J Pharm Sci. 2008; 70 (1): 31-5. [DOI:10.4103/0250-474X.40328]
6. Arabshahi-Delouee S, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 2007; 102 (4): 1233-40. [DOI:10.1016/j.foodchem.2006.07.013]
7. Aslanipour B, Heidari R, Farnad N. Phenolic Combination and Comparison of Antioxidant Activity in Three Different Alcoholic Extracts of Dracocephalum moldavica L. TURJAF. 2017; 5 (3): 199-206. [DOI:10.24925/turjaf.v5i3.199-206.812]
8. Barreira JCM, Ferreira ICFR, Olivera P, Pereira JA. Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem. 2007; 107 (3): 1106-13. [DOI:10.1016/j.foodchem.2007.09.030]
9. Baser KHC, Buchbauer G. Handbook of Essential Oils: Science, Technology, and Applications. Boca Raton, FL: CRC Press. 2015. [DOI:10.1201/b19393]
10. Be K, Gamlath S, Smith SC. In-vitro antimicrobial effect of spices on probiotic bacteria. Australas Medical J. 2010; 1: 113-40.
11. Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant sci. 2001; 161 (5): 839-51. [DOI:10.1016/S0168-9452(01)00490-3]
12. Cai S, Bay BH, Lee YK, Lu J. Mahendran R. Live and lyophilized Lactobacillus species elicit differential immunomodulatory effects on immune cells. FEMS Microbiol Lett. 2010; 302 (2): 189-96. [DOI:10.1111/j.1574-6968.2009.01853.x]
13. Chirinos R, Rogez H, Campos D, Pedreschi R, Larondelle Y. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruiz and Pavon tubers). Sep Purif Technol. 2007; 55 (2): 217-25. [DOI:10.1016/j.seppur.2006.12.005]
14. Dastmalchi K. Dracocephalum moldavica and Melissa officials: Chemistry and bioactivity relevant in Alzheimer disease therapy. Helsinki. 2008.
15. Falleh H, Ksouri R, Lucchessi ME, Abdell C, Magné C. Ultrasound-assisted extraction: effect of exraction time and solvent power on the levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Trop J Pharm Res. 2012; 11 (2): 243-9. [DOI:10.4314/tjpr.v11i2.10]
16. Kaska A, Deniz N, Çiçek M, Mammadov R. Evaluation of Antioxidant Properties, Phenolic Compounds, Anthelmintic, and Cytotoxic Activities of Various Extracts Isolated from Nepeta cadmea: An Endemic Plant for Turkey. J Food Sci. 2018; 83 (6): 1552-9. [DOI:10.1111/1750-3841.14167]
17. Kakasy AZ, Lemberkovics E, Kursinszki L, Janicsak G, Szoeke E. Data to the phytochemical evaluation of Pharm. Bull. 2004; 10: 1149-50.
18. Krasaekoopt W, Bhandari B, Deeth HC. Survival of probiotics encapsulated in chitosan-coated alginate beads in yoghurt from UHT- and conventionally treated milk during storage. LWT-Food Sci Technol. 2006; 39 (2): 177-83. [DOI:10.1016/j.lwt.2004.12.006]
19. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem. 2006; 97 (1): 109-14. [DOI:10.1016/j.foodchem.2005.03.032]
20. Mahmoudi R, Kazeminia M, Ghajarbeygi P, Pakbin B. An Introductory Review on Increasing the Survival of Probiotic Bacteria in Dairy Products Using Essential Oil. J Dent Oral Health. 2017; 3 (4): 1-4.
21. Marhamatizadeh MH, Ehsandoost E, Gholami P, Davanyan Mohaghegh M. Effect of Olive Leaf Extract on Growth and Viability of Lactobacillus acidophilus and Bifidobacterium bifidum for Production of Probiotic Milk and Yoghurt. Int J Farm Allied Sci. 2013; 2 (17): 572-8.
22. Mehrabani M, Roholahi S, Foroumadi A. Phytochemical studies of Dracocephalum polychaetum Bornm. J Med Plants. 2005; 4 (16): 36-42.
23. Modnicki D, Tokar M, Klimek B. Flavonoids and phenolic acids of Nepeta cataria L. var. citriodora (Becker) Balb. (Lamiaceae). Acta Pol Pharm. 2007; 64 (3): 247-52.
24. Naghibi F, Mosaddegh M, Mohamadi Motamed S. Labiatae family in folk medicine in Iran, from ethnobiology to pharmacology. Iran J Pharm Res. 2005; 2 (5): 63-79.
25. Oh NS, Lee JY, Joung JY, Kim KS, Shin YK, Lee KW, et al. Microbiological characterization and functionality of set-type yogurt fermented with potential prebiotic substrates Cudrania tricuspidata and Morus alba L. leaf extracts. J Dairy Sci. 2016; 99 (8): 6014-25. [DOI:10.3168/jds.2015-10814]
26. Parlatan A, Saricoban C, Ozcan MM. Chemical composition and antimicrobial activity of the extracts of kefe cumin (Laster trilobum l.) fruits from different regions. Int J food Sci Nutr. 2009; 60 (7): 606-17. [DOI:10.3109/09637480801993938]
27. Porto CD, Calligaris S, Cellotti E, Nicoli MC. Antiradical properties of commercial cognacs assessed by the DPPH test. J Agric Food Chem. 2000; 48 (9): 4241-5. [DOI:10.1021/jf000167b]
28. Pouraboli I, Nazari S, Sabet N, Sharififar F, Jafari M. Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies. Pharm Biol. 2016; 54 (2): 272-8. [DOI:10.3109/13880209.2015.1033561]
29. Saeidnia S, Gohari AR, Hadjiakhoondi A, Shafiee A. Bioactive compounds of the volatile oil of Dracocephalum kotschyi. Z Naturforsch C J Biosci. 2007; 62 (11-12): 793-6. [DOI:10.1515/znc-2007-11-1203]
30. Sarabi-Jamab M, Niazmand R. Effect of Essential Oil of Mentha piperita and Ziziphora clinopodioides on Lactobacillus acidophilus activity as bioyogurt starter culture American-Eurasian. J Agric Environ Sci. 2009; 6 (2): 129-31.
31. Simsek B, Sagdic O, Ozcelik S. Survival of Escherichia coli O157:H7 during the storage of Ayran produced with different spices. J Food Eng. 2007; 78 (2): 676-80. [DOI:10.1016/j.jfoodeng.2005.11.005]
32. Shah NP, Ding WK, Fallourd MJ, Leyer G. Improving the Stability of Probiotic Bacteria in Model Fruit Juices Using Vitamins and Antioxidants. J Food Sci. 2010; 75 (5): 278-82. [DOI:10.1111/j.1750-3841.2010.01628.x]
33. Shahdadi F. Qualitative and rheological properties of yogurt containing microencapsulated probiotic bacteria, PhD Thesis, Gorgan University of Agricultural Sciences and Natural Resources. 2015.
34. Shanaida M, Golembiovska O, Hudz N, Wieczorek P. Phenolic compounds of herbal infusions obtained from some species of the Lamiaceae family. Curr Issues Pharm Medical Sci. 2018; 31 (4): 194-9. [DOI:10.1515/cipms-2018-0036]
35. Taghizadeh M, Nasibi F, Manouchehri Kalantari K, Ghanati F. Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. Cell suspension culture under magnetite nanoparticles and static magnetic field elicitation. Plant Cell Tiss and Org Cult. 2019; 136 (3): 43-9. [DOI:10.1007/s11240-018-01530-1]
36. Turumtay A, İslamoğlu F, Çavuşa D, Şahin H, Turumtay H, Vanholme V. Correlation between phenolic compounds and antioxidant activity of Anzer tea (Thymus praecox) Opiz subsp. caucasicus var. caucasicus). Ind Crops Prod. 2014; 5 (3): 687-94. [DOI:10.1016/j.indcrop.2013.11.042]
37. Vahid Moghadam F, Mortazavi SA, Ghalemosiani Z. Antioxidant activity of aqueous extract of Marjoram and its effect on survival of Lactobacillus plantarum subspecies plantarum in low-fat probiotic yogurt. Food Sci Technol. 2018; 10 (1): 97-107.
38. Vamanu E, Nita S. Antioxidant Capacity and the Correlation with Major Phenolic Compounds, Anthocyanin, and Tocopherol Content in Various Extracts from the Wild Edible Boletus Edulis Mushroom. Biomed Res Int. 2013; 11 (3): 313-24. [DOI:10.1155/2013/313905]
39. Viana GS, Vale Furtado TG, Somotos EC. Central effect of citral, myrecene and limonene, constituents of essential oil chemotypes from Lippa Alba (mill) n.e. brown. Phytomedicine. 2000; 9 (8): 709-14. [DOI:10.1078/094471102321621304]
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.