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Golafrouz H, Ahari H, Anvar S A, Shahbazzadeh D. Detection of Staphylococcus aureus Enterotoxin A (SEA) Using Dot-ELISA in Milk Samples. JoMMID 2020; 8 (4) :132-136
URL: http://jommid.pasteur.ac.ir/article-1-232-en.html
Detection of Staphylococcus aureus Enterotoxin A (SEA) Using Dot-ELISA in Milk Samples
Haniyeh Golafrouz , Hamed Ahari , Seyed Amirali Anvar , Delavar Shahbazzadeh
Department of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (2190 Views)
Introduction: Staphylococcus aureus enterotoxin A (SEA) is one of the most common causes of staphylococcal food poisoning. Due to the simplicity and no requirement for laboratory apparatuses, dot-ELISA is a choice method for detecting Staphylococcal enterotoxins. The present study aimed to develop a dot-ELISA for the detection of SEA. Methods: Nitrocellulose membranes were coated with the SEA antibody and blocked by the addition of 3% bovine serum albumin (BSA) blocking buffer. After 1 h incubation and washing the membranes, milk samples and the positive control (SEA, 50 ng/ml) were added to the membranes and incubated for 1 h. The membranes were then washed and incubated for 45 min with HRP-conjugated SEA, followed by the addition of TMB. Results: Our dot-ELISA could detect amounts of ≥ 50 ng/ml of SEA in the milk samples. Of the 30 raw milk samples randomly purchased from dairy product stores in District 3, Tehran, 5 (16%) contained SEA ≥ 50 ng/ml by the dot-ELISA. Conclusion: The dot-ELISA showed to be a reliable method for the preliminary screening of milk samples for SEA contamination. This method is cost-effective, fast, and does not require an ELISA-reader device.
Keywords: dot-ELISA, Staphylococcus aureus Enterotoxin A (SEA), Raw milk
Full-Text [PDF 810 kb]   (1136 Downloads)    
Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2020/01/17 | Accepted: 2020/10/19 | Published: 2021/02/13
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.