Volume 6, Issue 2 And 3 (4-2018)                   JoMMID 2018, 6(2 And 3): 53-56 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Farahnak A, Zaki L, Molaei Rad M B, Golestani A, Eshraghiyan M R. Increased Alkaline Phosphatase Activity in the Somatic Extract of Hydatid Cyst Protoscoleces upon Treatment with Albendazole, an Implication for in vitro Evaluation of Drug Efficiency. JoMMID 2018; 6 (2 and 3) :53-56
URL: http://jommid.pasteur.ac.ir/article-1-167-en.html
Increased Alkaline Phosphatase Activity in the Somatic Extract of Hydatid Cyst Protoscoleces upon Treatment with Albendazole, an Implication for in vitro Evaluation of Drug Efficiency
Ali Farahnak * , Leila Zaki , Mohamad Bagher Molaei Rad , Abolfazl Golestani , Mohammad Reza Eshraghiyan
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:   (4796 Views)
Introduction: Hydatidosis is an endemic parasitic disease of humans in Iran, and Albendazole (ABZ) is a drug of choice for treatment of this infection. As the Alkaline phosphatase (ALP) is necessary for the metabolism of parasites, this study was aimed to evaluate the effect of ABZ on ALP enzyme activity in hydatid cyst parasite as a marker for drug efficiency. Methods: In the present study, the ALP activity level was estimated in the extracts of the untreated parasite (Hydatid cyst protoscoleces) as well as the ABZ-treated samples with a final concentration of 100 µg. The protein concentration and the protein bands in the extracted samples were analyzed by Bradford and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods, respectively. Results: The results showed that the mean value of the ALP activity level of the treated samples (0.474 U/ml/mg) was significantly higher than that of untreated samples (0.205 U/ml/mg) (P<0.05). SDS-PAGE analysis demonstrated the higher intensity of the 59 kDa protein band in ABZ-treated samples, compared to the untreated sample. Conclusion: Considering the effect of the ABZ drug on ALP activity in the hydatid cyst protoscoleces, this enzyme might be regarded as an indicator for the effectivity of drug on this parasite.
Keywords: Albendazole, Parasites, Alkaline Phosphatase, Hydatid cyst, Iran
Full-Text [PDF 191 kb]   (2381 Downloads)    
Type of Study: Original article | Subject: Other
Received: 2018/06/19 | Accepted: 2018/08/1 | Published: 2019/03/18
References
1. Pang N, Zhang F, Li S, Zhu Y, Zhang C, An M, et al. TGF-β/Smad signaling pathway positively up-regulates the differentiation of Interleukin-9-producing CD4+ T cells in human Echinococcus granulosus infection. J Infect. 2018; 76 (4): 406-16. [DOI:10.1016/j.jinf.2018.01.005]
2. Fasihi Harandi M, Budke CM, Rostami S. The Monetary Burden of Cystic Echinococcosis in Iran. PLOS Negl Trop Dis. 2012; 6 (11): e1915. [DOI:10.1371/journal.pntd.0001915]
3. Saimot AG, Meulemans A, Cremieux AC, Giovanangeli MD, Hay JM, Delaitre B, et al. Albendazole as a potential treatment for human hydatidosis. Lancet. 1983; 322 (8351): 652-6. [DOI:10.1016/S0140-6736(83)92533-3]
4. Franchi C, Di Vico B, Teggi A. Long-term evaluation of patients with hydatidosis treated with benzimidazole carbamates. Clin Infect Dis. 1999; 29 (2): 304-9. [DOI:10.1086/520205]
5. Conchedda M, Bortoletti G, Ecca AR, Gabriale F, Palmas C. Study on immunobiology in endoparasites of public health interest: Echinococcosis-Hydatidosis. Parasitologia. 2001; 43: 11-9.
6. Stoyanov G, Yaramov N, Damianov N, Bekova P. Enzymology of the liver in hydatidosis. Khirurgiia (Sofiia). 2010; (6): 40-1 [In Bulgarian].
7. Bhardwaj R, Skelly PJ. Characterization of Schistosome Tegumental Alkaline Phosphatase (SmAP). PLoS Negl Trop Dis. 2011; 5 (4): 1011-9. [DOI:10.1371/journal.pntd.0001011]
8. Liquori GE, Mastrodonato M, Rossi R, Scillitani G, Gena P, Portincasa P, et al. Altered membrane glycoprotein targeting in cholestatic hepatocytes. Eur J Clin Invest. 2010; 40 (5): 393-400. [DOI:10.1111/j.1365-2362.2010.02273.x]
9. Waghmare SB, Chavan RJ. Some quantitative studies of carbohydrate metabolites in cestode parasite of Gallus gallus domesticus. Int J Parasitol. 2010; 2 (1): 1-4. [DOI:10.9735/0975-3702.2.1.1-4]
10. Adnani sadati SJ, Farahnak A, Molaei rad MB, Golestani A, Eshraghiyan MR. A Comparison between the Effects of Albendazole and Mebendazole on the Enzymatic Activity of Excretory/Secretory Products of Echinococcus granulosus Protoscoleces in Vitro. Iran J Public Health. 2016; 45 (2): 223-9.
11. Maizels RM, Blaxter ML, Robertson BD, Selkirk ME. Parasite antigens, parasite genes: a laboratory manual for molecular parasitology. Cambridge University Press: Imperial College of Science. Technology and Medicine; 1991; 1-27.
12. Swarna SR, Parija SC. Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis. Rev Inst Med Trop Sao Paulo. 2008; 50 (4): 233-6. [DOI:10.1590/S0036-46652008000400009]
13. Dalimi A, Shamsi M, Khosravi A, Ghaffarifar F. Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran. Iran J Parasitol. 2017; 12 (4): 614-21.
14. Yin J, Liu C, Shen Y, Zhang H, Cao J. Efficacy of ursolic acid against Echinococcus granulosus in vitro and in a murine infection model. Parasit Vectors. 2018; 11 (1): 58. [DOI:10.1186/s13071-018-2628-8]
15. Cowan N, Meier C, Neodo A, Keiser J. Exposure of Heligmosomoides polygyrus and Trichuris muris to albendazole, albendazole sulfoxide, mebendazole and oxantel pamoate in vitro and in vivo to elucidate the pathway of drug entry into these gastrointestinal nematodes. Int J Parasitol Drugs Drug Resist. 2017; 7 (2): 159-73. [DOI:10.1016/j.ijpddr.2017.03.005]
16. Senyuz OF, Yesildag E, Celayir S. Albendazole therapy in the treatment of hydatid liver disease. Surg Today. 2001; 31 (6): 487-91. [DOI:10.1007/s005950170106]
17. Saimot AG. Medical treatment of liver hydatidosis. World J Surg, 2001; 25 (1): 15-20. [DOI:10.1007/s002680020003]
18. Horton R. Albendazole in treatment of human cystic echinococcosis: 12 years of experience. Acta Trop. 1997; 64 (1-2): 79-93. [DOI:10.1016/S0001-706X(96)00640-7]
19. Polat E, Aslan M, Cakan H, Saribas S, Ipek T, Kocazeybek B. The effects of albendazole and povidone ıodine for hydatid cysts protoscoleces, ın-vitro and in-vivo. Afr J Microbiol Res. 2009; 3 (11): 743-6.
20. Adas G, Arikan S, Kemik O, Oner A, Sahip N, Karatepe O. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol. 2009; 15 (1): 112-16. [DOI:10.3748/wjg.15.112]
21. Xiao SH, Feng JJ, Guo HF, Jiao PY, Yao MY, Jiao W. Effects of mebendazole, albendazole, and praziquantel on fumarate hydratase, pyruvate kinase, and phosphoenolpyruvate carboxykinase of Echinococcus granulosus cyst wall harbored in mice. Zhongguo yao Li Xue Bao. 1994; 15(1): 69-72.
22. Virginio VG, Monteiro KM, Drumond F, de Carvalho MO, Vargas DM, Zaha A, et al. Excretory/secretory products from in vitro-cultured Echinococcus granulosus. Mol Biochem Parasitol. 2012; 183 (1): 15-22. [DOI:10.1016/j.molbiopara.2012.01.001]
23. Farrokhi Karibozorg M, Farahnak A, Molaei Rad MB, Golmohammadi T, Eshraghian MR. Assessment of Alkaline Phosphatase Activity in Hydatid Cyst Protoscolices and Liver Tissue as a Pathological Biomarker. JoMMID. 2014; 2 (2) :68-70.
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.