Volume 5, Issue 3 And 4 (7-2017)                   JoMMID 2017, 5(3 And 4): 47-50 | Back to browse issues page

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Tabasi M, Azizian R, Eskandarion M R, Habibi M, Asadi Karam M R. Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran. JoMMID 2017; 5 (3 and 4) :47-50
URL: http://jommid.pasteur.ac.ir/article-1-157-en.html
Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran
Mohsen Tabasi , Reza Azizian , Mohammad Reza Eskandarion , Mehri Habibi , Mohammad Reza Asadi Karam *
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (5641 Views)
Introduction: The strains of Pseudomonas aeruginosa are known as an opportunistic pathogen that can cause infections in humans and animals. Metallo-β-lactamases (MBLs) are the most significant factors of resistance to carbapenem antibiotics in these bacteria. This study was designed to identify the MBLs producing P. aeruginosa isolates in three hospitals of Tehran, Iran. Methods: Totally, we obtained 665 samples from patients hospitalized in three hospitals in Tehran, Iran. Antibiotic-susceptibility test of the P. aeruginosa isolates was done based on Kirby-Bauer disk diffusion test. The Minimum Inhibitory Concentration (MIC) of the isolates was performed using agar dilution method, and IPM-EDTA test identified MBL producing isolates. Results: Among the examined isolates, 473 (71.1%) were P. aeruginosa. Among these, 306 (64.7%) were resistant to imipenem, and 289 (94.5%) were MBL producers. Furthermore, the resistance rate of the isolates to other antibiotics was amikacin (26%), tobramycin (24.95%), ceftazidime (23.05%), gentamicin (22.83%), carbenicillin (21.14%), and ceftizoxime (18.19%). The MICs of imipenem and ceftazidime for the majority of the isolates were 4 µg/ml and ˃128 µg/ml, respectively. Conclusion: This study confirmed previous reports on the increased rate of MBL-mediated resistance in P. aeruginosa isolates worldwide. Therefore, detection of resistance patterns for these isolates, particularly MBLs, is necessary for prevention and control of Pseudomonas associated infections.
Keywords: Pseudomonas aeruginosa, Metallo-β-lactamase, IPM-EDTA, MIC
Full-Text [PDF 223 kb]   (2362 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2018/03/5 | Accepted: 2018/03/14 | Published: 2018/04/18
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.