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Sarreshteh E, Rostamian M, Tat Asadi M, Abrishami F, Najafi A, Abolghazi M et al . Route of Infection Affects Pathogenicity of Leishmania major in BALB/c Mice . JoMMID 2017; 5 (1 and 2) :26-30
URL: http://jommid.pasteur.ac.ir/article-1-141-en.html
Route of Infection Affects Pathogenicity of Leishmania major in BALB/c Mice
Ehsan Sarreshteh , Mosayeb Rostamian , Mahsa Tat Asadi , Firoozeh Abrishami , Ali Najafi , Maryam Abolghazi , Hamid Mahmoudzadeh Niknam *
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (5679 Views)
Introduction: The term Leishmaniasis applies to a group of parasitic diseases caused by the genus Leishmania. Route of infection is one of the variables that have been reported to influence the immune responses as well as the disease outcome in experimental models of leishmaniasis. This research aims to study the effect of route of infection on the pathogenicity of Leishmania major in BALB/c mice. Methods: Low (103 parasites/mouse) and high (106 parasites/mouse) doses of L. major was injected into footpad or ear dermis of BALB/c mice. Lesion diameter was determined throughout the study. Parasite load of draining lymph nodes and spleen were assessed at three intervals. Results: Footpad in comparison to ear route showed higher pathogenicity of L. major in BALB/c mice as assessed by lesion diameter, parasite load in the draining lymph node, and dissemination of the parasite to the spleen. Conclusion: Our findings suggest that substantial differences between footpad and ear route need particular attention when we use experimental models for the study of Leishmania infections.
Keywords: Leishmania major, Pathogenicity, Administration Routes, Viscera, BALB/c mice
Full-Text [PDF 370 kb]   (1688 Downloads)    
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2017/09/17 | Accepted: 2017/12/25 | Published: 2018/01/17
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.