Volume 4, Issue 3 And 4 (7-2016)                   JoMMID 2016, 4(3 And 4): 68-75 | Back to browse issues page

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Faezi Ghasemi M, Alikhani F. The Impact of Overexpression of Sigma Factors on Morphological Changes, Growth Pattern, and Biofilm Formation in Mycobacterium marinum CCUG 20998. JoMMID. 2016; 4 (3 and 4) :68-75
URL: http://jommid.pasteur.ac.ir/article-1-121-en.html
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
Abstract:   (2742 Views)
Introduction: Bacteria have at least one sigma factor (σ-factor) that transcribes the genes required for cell viability. Usually, transcription of σ-factors occurs and changes in response to a variety of environmental stresses. Expression of σ-factors is one of the strategies which is used in response to different stress conditions. This study was aimed to evaluate the effects of overexpression of σ-factors genes including σA, σB, σD, σE, σF, σG, σH, σJ, σK, σL, and σM on morphology, growth pattern and biofilm formation in Mycobacterium marinum CCUG 20998. Methods: In this study, the genes for major σ-factors were cloned in the expression vector pAGHD1, containing 11 kb Hind III fragment of pAG1 and Tetz determinants. A quantitative real-time PCR (qRT-PCR) assay was used to quantify σ-factor mRNA levels of σ-factors in exponential and stationary phases. The overexpression in real-time experiments was normalized to the σA expression level. The effect of expression was evaluated on biofilm formation in this bacterium. Results: Some selected σ-factors used in this study were overexpressed. The σB had the highest expression level during the exponential and stationary phases. The σ-factors σD, σG, and σH showed lower expression level compared with σE, σF, and σK. The lowest expression belonged to σL and σM σ-factors. Also, overexpression of σE and σK led to more biofilm formation in comparison to other σ-factors in M. marinum CCUG 20998. Conclusion: The overexpression of some σ-factors can affect growth, morphology and biofilm formation in M. marinum CCUG 20998.
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Type of Study: Original article | Subject: Other
Received: 2017/05/4 | Accepted: 2017/07/24 | Published: 2017/09/11

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