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Faisal Shahbaz Akram M, Ashraf M, Ali S, Kazmi S I. Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties. JoMMID 2017; 5 (1 and 2) :12-16
URL: http://jommid.pasteur.ac.ir/article-1-106-en.html
Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties
Muhammad Faisal Shahbaz Akram , Muhammad Ashraf , Sultan Ali , Syeda Iqra Kazmi
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
Abstract:   (5655 Views)
Introduction: Probiotics are defined as live non-pathogenic microorganisms and if administered in adequate amounts, can have beneficial effects on their hosts. This study aimed to isolate Gram-positive bacteria from different sources and to evaluate their probiotic properties. Methods: We obtained five samples from various sources including yogurt, infant feces, cheese, and soil under aseptic methods. The samples were inoculated on MRS agar and M17 medium. Biochemical assays identified isolated bacteria. The probiotic effect of isolated bacteria was tested by applying acidic pH 2.0, 0.3% w/v bile salt concentration and gastric juice in the samples. The antibiotic susceptibility profiling and their antimicrobial effects against Escherichia coli, Staphylococcus aureus, Salmonella enterica and Pseudomonas aeruginosa, were determined. Results: Four Gram-positive bacteria including Lactobacillus acidophilus, Bifidobacterium bifidum, Lactococcus lactis and Bacillus subtilis were isolated from yogurt, infant feces, cheese, and soil, respectively. Except for B. subtilis, all the isolated bacteria were catalase-negative and non-motile. The results showed that viable count of L. acidophilus, B. bifidum, and L. lactis was significantly high (P<0.05), and B. subtilis was sensitive. The isolates showed an antimicrobial effect against all indicator pathogens except P. aeruginosa. The isolated B. subtilis showed no antimicrobial effect. The antibiotic susceptibility was determined for ten different antibiotics. All the isolates were susceptible to most of the antibiotics (table 6) and resistant to penicillin G, gentamicin, vancomycin, and kanamycin. Conclusion: The species L. acidophilus, B. bifidum, and L. lactis fulfilled the criteria for probiotic properties and can be used in field conditions as probiotics.
Keywords: Identification, Gram-Positive Bacteria, Probiotic
Full-Text [PDF 363 kb]   (3567 Downloads)    
Type of Study: Original article | Subject: Other
Received: 2016/11/6 | Accepted: 2017/06/11 | Published: 2018/01/15
References
1. Ashraf M, Arshad M, Siddique M, Muhammad G. In vitro screening of locally isolated Lactobacillus species for probiotic properties. Pak Vet J. 2009; 29 (4): 186-90.
2. Reid G, Sanders ME, Gaskins HR, Gibson GR, Mercenier A, Rastall RA, Roberfroid MB, Rowland I, Cherbut C, Klaenhammer TR. New scientific paradigms for probiotics and prebiotics. J Clin Gastroenterol. 2003; 37 (2): 105-18. [DOI:10.1097/00004836-200308000-00004] [PMID]
3. Pundir RK, Rana S, Kashyap N, Kaur A. Probiotic potential of lactic acid bacteria isolated from food samples an in vitro study. J Appl Pharm Sci. 2013; 3 (3): 85-93.
4. Chiang SS, Pan TM. Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products. Appl Microbiol Biotechnol. 2012; 45: 903-16. 93 (3): 903-16.
5. Behnsen J, Deriu E, Sassone-corsi M, Raffatellu M. Probiotics properties, examples, and specific applications. Cold Spring Harb Perspect Med. 2013; 3 (3): a10074. [DOI:10.1101/cshperspect.a010074] [PMID] [PMCID]
6. Guo XH, Kim JM, Nam HM, Park SY, Kim JM. Screening lactic acid bacteria from swine origins for multi strain probiotics based on in vitro functional properties. Anaerobe. 2010; 16 (4): 321-6. [DOI:10.1016/j.anaerobe.2010.03.006] [PMID]
7. Tambekar DH, Bhutada SA. An evaluation of probiotic potential of Lactobacillus spp from milk of domestic animals and commercial available probiotic preparations in prevention of enteric bacterial infections. Recent Res Sci Technol. 2010; 2 (10): 82-8.
8. Mombelli B, Gismondo MR. The use of probiotics in medical practice. Int J Antimicrob Agents. 2000; 16 (4): 531-6. [DOI:10.1016/S0924-8579(00)00322-8]
9. Savadago A, Ouattara CAT, Bassole IHN, Traore SA. Bacteriocins and lactic acid bacteria a mini reviews. Afr J Biotechnol. 2006; 5 (9): 678-83.
10. Oluwajoba SO, Akinyosoye FA, Oyetayo VO. In Vitro Screening and Selection of Probiotic Lactic Acid Bacteria Isolated from Spontaneously Fermenting Kunu-Zaki. Adv Microbiol. 2013; 3(4): 309-16. [DOI:10.4236/aim.2013.34044]
11. Zhang ZY, Liu C, Guo XK. Research progress of antibiotics resistance in lactic acid bacteria. Chinese Journal of Microecology. 2007; 19 (5): 478 [In Chinese].
12. Ali FS, Saad OAO, Hussein SA. Antimicrobial activity of probiotic bacteria. Egypt Acad J Biol Sci. 2013; 5 (2): 21-34.
13. Galvez A, Abriouel H, Lopez RL, Omar NB. Bacteriocin based strategies for food bio preservation. Int J Food Microbial. 2007; 120 (1): 51-70. [DOI:10.1016/j.ijfoodmicro.2007.06.001] [PMID]
14. Leal-sanchez M, Ruiz-Barba JL, Sanchez AH, Rejano L, Jimenez-Diaz R, Garrido A. Fermentation profile and optimization of green line fermentation using Lactobacillus plantarum LPCO1O as starter culture. Food Microbiol. 2003; 20 (4): 421-30. [DOI:10.1016/S0740-0020(02)00147-8]
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.