en Microbial pathogenesis Microbial pathogenesis Original article Original article <b><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> Bacterial vaginosis (BV) is the most common vaginitis in childbearing women. Despite the availability of various treatments, recurrence rates are high. This study investigated the effects and side effects of vitamin C vaginal tablets in patients with <i>Gardnerella vaginalis</i> infection based on Amsel criteria and the culture. <b>Methods:</b> A double-blind, randomized clinical trial was conducted on 48 non-pregnant women aged 15-40 years referred to Alawi Hospital in Ardabil Province, Iran. After the diagnosis of <i>G. vaginalis</i> infection based on Amsel criteria and culture methods, the patients were randomly assigned to receive either intravaginal 250 mg vitamin C (intervention group, n=24) or 250 mg metronidazole tablets (control group, n=24) for eight nights. The patients were evaluated 10 &plusmn; 2 and 30 &plusmn; 2 days before and after the treatment. <b>Results:</b> Culture and Amsel criteria showed significant improvement in both groups during the first and fourth weeks of treatment. However, the intervention group that received vitamin C showed a more substantial improvement (<i>P</i><0.002, <i>P</i><0.001). Intravaginal vitamin tablets were more effective than metronidazole tablets in treating <i>G. vaginalis</i> infection. Furthermore, vitamin C tablets improved the abnormal growth of vaginal microflora and reduced the abnormally high pH of the vagina. This treatment effectively prevented the recurrence of <i>G. vaginalis</i> infection for one month.<b> Conclusion</b>: Vitamin C vaginal tablets showed to be a promising alternative treatment for <i>G. vaginalis</i> infection, but further research is needed to confirm these findings and address potential limitations.</span></span></span> Gardnerella vaginalis, Vitamin C vaginal tablets, Amsel, Bacterial vaginosis, Treatment, Intravaginal, Diagnosis, Lactobacillus, Probiotics 168 173 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-362-3&slc_lang=en&sid=1 Golsuom Sourazur s.sorazar@mail.com 0000-0001-6021-0067 Yes بیمارستان علوی اردبیل