en Epidemiologic studies including microbial genotyping, phenotyping and serotyping Epidemiologic studies including microbial genotyping, phenotyping and serotyping Original article Original article <strong>Introduction</strong>: Crimean-Congo Hemorrhagic Fever (CCHF) is a zoonotic viral infection transmitted mainly via CCHF virus-infected ticks between vertebrate hosts. The disease occurs in almost all provinces of Iran. This study investigated the CCHFV infection in hard ticks collected from livestock in the Sistan region of Sistan and Baluchestan Province, southeast of Iran. <strong>Methods:</strong> In this study, ticks were collected from 220 livestock, including 150 sheep, 50 goats, 20 cows in five counties of Sistan Province (Zabol, Zehak, Hirmand, Nimruz, and Hamun). The ticks were identified under a stereomicroscope according to valid morphological keys. A reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the CCHFV genome via amplifying the S segment. <strong>Results:</strong> Among 100 selected ticks, RT-PCR revealed no CCHFV infection. <strong>Conclusions:</strong> Although no ticks were positive for CCHFV, it should be recalled that Sistan and Baluchestan province is among the highly endemic CCHF foci. As a result, further investigation and larger sample sizes are required to confirm our outcome. According to the hypothesis that direct contact with viremic livestock is more significant than tick bites in the viral transmission, more serological and molecular screening should be performed on high-risk individuals, e.g., slaughterhouse staff, ranchers, farmers, and veterinarians in the Sistan region. Crimean-Congo hemorrhagic fever, Hard tick, RT-PCR, Sistan, Iran 30 35 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-359-1&slc_lang=en&sid=1 Sahar Asadolahizoj dr.sahar.asd@gmail.com 0000-0002-4459-4248 No DVM graduated, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. Dariush Saadati saadatdariush@uoz.ac.ir 0000-0001-9002-1507 Yes Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Mehdi Rasekh mrasekh@ut.ac.ir 0000-0003-1505-6640 No Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Faezeh Faghihi faezefaghihi@yahoo.com 0000-0003-1657-6242 No Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran Mehdi Fazlalipour M_fazlalipour@pasteur.ac.ir 0000-0001-9367-6955 No Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran Amir Sajad Jafari a.sajad.jafari@gmail.com 0000-0002-6830-6706 No Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran