en Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article <table class="Table" style="width:101.14%; border-collapse:collapse" width="101%"> <tbody> <tr> <td style="border-bottom:1px solid black; width:69%; padding:0in 7px 0in 7px; height:24px; border-top:1px solid black; border-right:none; border-left:none" valign="top"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> In recent years, the green synthesis of nanoparticles has received much attention. Green synthesis has several advantages over other methods: cost-effectiveness, simplicity, and non-toxicity. In the present study, we obtained the aqueous extract of <i>Thymus daenensis</i> (Celak) flora, biosynthesized the copper nanoparticles (Cu-NPs), and evaluated the antifungal activity. <b>Methods:</b> UV-vis spectroscopy analyses, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) were used to identify the synthesized nanoparticles. The antifungal activity of the synthesized copper nanoparticles was evaluated using the microdilution method. <b>Results:</b> After adding the extract to the copper sulfate solution, the solution color changed from light blue to yellowish-green. A maximum peak at the wavelength of 414 nm confirmed the copper nanoparticles formation. Scanning electron microscopy demonstrated the particle size ranging from 30 nm to 42 nm. The biosynthesized Cu-NPs had an inhibitory effect against <i>Candida albicans</i>, <i>Fusarium solani</i>, <i>Aspergillus Niger</i>, and <i>Aspergillus flavus</i>. <b>Conclusion:</b> Our findings demonstrated that <i>T. daenensis</i> aqueous extract acts as a reducer and stabilizer factor. We successfully synthesized Cu-NPs from copper sulfate using <i>T. daenensis</i> (Celak) flora aqueous extract according to the UV-Vis spectrum, FTIR, and SEM results. This research was the first report of Cu-NPs synthesized from an aqueous <i>T. daenensis</i> (Celak) flora extract. Our simple, quick, and inexpensive method for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.</span></span></span></span></span></td> </tr> </tbody> </table> Antifungal activity, Aqueous extract, Green synthesis, Copper nanoparticles 98 103 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-302-1&slc_lang=en&sid=1 Katrin Ebrahimi ebr_k@yahoo.com 0000-0001-5595-8425 Yes Biology Group, Payam Noor University, Tehran, Iran Sima Shir Ovand sima.shiravand@gmail.com 0000-0002-9987-4407 No Biology Group, University of Lorestan Abd al Nasser Mohammedi Mohammadi.ab@lu.ac.ir 0000-0001-7769-6438 No Mycology Department, University of Lorestan Mohsen Nabi-Afjadi mohsennabi66@gmail.com 0000-0003-4573-5337 No Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Hamidreza Zalpoor 0000-0002-8057-2804 No Network of Immunity in Infection, Malignancy & Autoimmunity (NIIMA), Universal Scientific Education & Research Network (USERN), Tehran, Iran Farbod Bahreini 0000-0003-0884-2029 No Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran