Journal of Medical Microbiology and Infectious Diseases Journal of Medical Microbiology and Infectious Diseases JoMMID Medical Sciences http://jommid.pasteur.ac.ir 1 admin 2345-5349 2345-5330 8 10.61882/JoMMID 14 8888 13 en jalali 1399 12 1 gregorian 2021 3 1 9 1 online 1 fulltext
en The Effect of Silibinin on the Expression of TLR7, ISG15, and SOCS1 in Peripheral Blood Mononuclear Cells of Hepatitis C Infected Patients in Comparison with Interferon-α Infectious diseases and public health Infectious diseases and public health Original article Original article <strong>Introduction: </strong>Silibinin (silibinin A) is the most active silymarin component, which acts both as a hepatoprotective [1] and an antiviral agent. The present study investigated the silibinin effect on IFN-related innate immune genes in PBMCs from HCV-infected patients.<strong> Method:</strong> 22 chronic HCV patients, including 10 IFN responders and 12 non-responders, were included. Their isolated PBMCs were treated for 6 hours in the presence of silibinin, IFN-&alpha;, or their combination. The transcription level of TLR7, ISG15, and SOCS1 genes was compared using real-time PCR.<strong> Result:</strong> Our result showed that IFN-&alpha; induced a significant up-regulation of TLR7 and ISG15 in PBMCs of both responder and non-responder groups. Nevertheless, the SOCS1 gene was not significantly changed in the non-responder group (P=0.32). The combination of IFN&alpha;<span dir="RTL">-</span> and silibinin showed a similar pattern to IFN-&alpha; alone. By itself, silibinin did not leave a significant change on the expression level of the studied genes<span dir="RTL">. </span><strong>Conclusion:</strong> The results indicated that silibinin did not enhance or suppress the expression level of TLR7, ISG15, and SOCS1 genes.&nbsp; Therefore, it has been suggested that its anti-inflammatory role might be devoid of IFN pathways. HCV, Silibinin, Interferon, ISG15, SOCS1 12 16 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-271-1&slc_lang=en&sid=1 Razieh Dowran raziehdowran@yahoo.com 0000-0002-2318-4397 No Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Seyed Younes Hosseini yovirus2002@yahoo.com 0000-0002-5881-6796 No Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Mohammad Reza Fattahi fattahim@sums.ac.ir 0000-0001-6160-0403 No Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran Jamal Sarvari sarvarij@sums.ac.ir 0000-0002-2259-0836 Yes Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran