Journal of Medical Microbiology and Infectious Diseases Journal of Medical Microbiology and Infectious Diseases JoMMID Medical Sciences http://jommid.pasteur.ac.ir 1 admin 2345-5349 2345-5330 8 10.61882/JoMMID 14 8888 13 en jalali 1398 10 1 gregorian 2020 1 1 8 1 online 1 fulltext
en The Effects of Ellagic Acid on Growth and Biofilm Formation of Candida albicans Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article <strong>Introduction: </strong>Biofilm formation is one of the specific features of <em>Candida albicans</em> that protects it from antifungal agents and the host immune system. Also, Biofilm formation by <em>C. albicans</em> on the mucosal surfaces and medical devices are responsible for causing <em>Candida</em> nosocomial infection. Here, we investigated the effects of ellagic acid on <em>C. albicans</em> growth and biofilm formation regarding the expression of two essential genes that are involved in adhesion and yeast-hypha transition. <strong>Methods:</strong> The yeasts were treated with serial two-fold concentrations of ellagic acid (3.125-100 &micro;g/ml) for 48 h at 35&deg;C. The weights of the cultured yeasts were measured as an indicator of the fungal growth, and the biofilm formation was assessed by a tetrazolium salt (XTT) reduction assay. The expressions of <em>HWP1</em> and <em>ALS3</em> genes were assayed by real-time PCR. <strong>Results:</strong> Ellagic acid inhibited <em>C. albicans</em> growth 0.68%-82.44%, dose-dependently. The biofilm formation also reduced 2.61%-68.318%. Also, The expressions of <em>HWP1</em> and <em>ALS3</em> genes were notably suppressed by ellagic acid at different concentrations. <strong>Conclusion:</strong> Our results showed that ellagic acid is a potential candidate to eliminate <em>C. albicans</em>-generated biofilm by suppressing the expression of the involved genes. Ellagic acid, Candida albicans, Biofilm, Gene 14 18 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-131-1&slc_lang=en&sid=1 Sepideh Nejatbakhsh baharnb181991@yahoo.com 0000-0002-0517-235X No Department of Mycology, Pasteur Institute of Iran, Tehran, Iran Mohsen Ilkhanizadeh-Qomi mohsenilkhani3@gmail.com 0000-0002-2036-5221 No Department of Mycology, Pasteur Institute of Iran, Tehran, Iran Mehdi Razzaghi-Abyaneh 0000-0001-8217-399X No Department of Mycology, Pasteur Institute of Iran, Tehran Zahra Jahanshiri zjahanshiri@gmail.com 0000000194319335 Yes Department of mycology, Pasteur Institute of Iran, Tehran, Iran