Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1398
4
1
gregorian
2019
7
1
7
3
online
1
fulltext
en
Comparison of Cysteine Protease B Gene Expression between Clinical Isolates of Leishmania tropica, Leishmania major and Leishmania infantum
Microbial pathogenesis
Microbial pathogenesis
Original article
Original article
<strong>Introduction: </strong><em>Leishmania</em> cysteine protease B (CPB) is a parasite virulence factor that plays a vital role in host-parasitic interactions. Regarding the importance of the CPB gene, we used a quantitative real-time RT-PCR to investigate the expression of CPB in the Iranian isolates of different <em>Leishmania </em>species. <strong>Methods:</strong> In this study, 36 clinical samples were collected, out of which 29 belonged to cutaneous leishmaniasis (CL), 3 to viscerotropic leishmaniasis (VTL), and 5 to visceral leishmaniasis (VL) patients. Among CL isolates, 8 were <em>Leishmania major</em>, and 21 were <em>Leishmania tropica, </em>including 3 isolates from the lupoid type. After RNA extraction and cDNA synthesis, gene expression analysis was performed by qPCR. <strong>Results:</strong> Our results showed the highest expression of CPB in isolates of <em>Leishmania infantum</em>, followed by <em>L. major</em> and <em>L. tropica</em>. Among <em>L. tropica</em> isolates, in the lupoid forms, the mean expression of CPB was ≈6.4 times higher than that of non-lupoid isolates. In <em>L. major</em> isolates, a significant difference was observed between the level of gene expression and the duration of the infection. The expression level of CPB was associated with the severity of the infection in <em>L. infantum </em>isolates. <strong>Conclusion:</strong> The CPB gene expressed in all<em> Leishmania </em>species<em>. </em>The highest expression was in <em>L. infantum</em> species and the least expression in <em>L. tropica</em>. The transcript level of CPB increased in <em>L. major</em> isolates derived from patients with a higher number and duration of ulcers; however, further studies on more clinical samples are needed to explore our findings.
Cysteine protease B (CPB), Real-time RT- PCR, Leishmania species, Iran
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http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-211-1&slc_lang=en&sid=1
Elham
Kazemirad
elham.kazemirad@gmail.com