en Original article Original article <strong>Introduction:</strong> Urinary tract infection is a common nosocomial infection that has recently become difficult to treat because of the increased emergence of multi-drug resistant strains. This study aims to determine the minimum inhibitory concentration, and molecular pattern of resistance to fosfomycin in <em>Escherichia</em> <em>coli</em> isolates originated from patients hospitalized with urinary tract infection in the intensive care unit (ICU) and coronary care unit (CCU) in three hospitals of Gorgan, northeast of Iran. <strong>Methods: </strong>Urine samples were obtained from 106 patients in three hospitals of Gorgan, northeast of Iran. After isolation and identification of <em>E. coli</em> isolates, the Kirby-Bauer disk diffusion test was performed to evaluate the antibiotic susceptibility pattern. Minimum inhibitory concentrations (MICs) of isolates to fosfomycin were determined using the agar dilution method over a concentration range of 0.5-1024 &mu;g/mL. Also, the presence of <em>murA</em> and <em>glpT</em> genes were investigated using polymerase chain reaction with specific primers. <strong>Results:</strong> Frequency of <em>E. coli</em> isolates was 62.3%, most of which originated from ICU patients (56.5%). The rate of susceptibility to fosfomycin was 85%. Moreover, the MIC of 80.3% of the isolates was less than or equal to 64 &mu;g/mL. We also detected the <em>murA</em> and <em>glpT</em> genes in 77.8% and 22.2% of fosfomycin-resistant isolates, respectively. <strong>Conclusion:</strong> Our results indicated a high bactericidal activity of fosfomycin against uropathogenic <em>E. coli</em> isolates. In agreement with similar studies, we concluded that the presence of <em>murA</em> is significantly associated with the development of resistance to fosfomycin. Fosfomycin, Uropathogenic Escherichia coli, Coronary Care Units, PCR 112 117 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-196-1&slc_lang=en&sid=1 Sara Malekpour Kolbadinezhad. saramalekpour@yahoo.com 0000-0001-9946-1031 No Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran Leila Fozouni lili_kia@yahoo.com 0000-0002-2248-0354 Yes Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran