en Review article Review article Rabies is a zoonotic viral disease. The causative agent is a negative-sense RNA genome virus of the genus <em>Lyssavirus</em> (Family: <em>Rhabdoviridae</em>). The disease, commonly transmitted by rabid dogs, is the cause of mortality of over 59000 humans worldwide annually. This disease can be prevented before the development of symptoms through proper vaccination even after exposure. Hence, improvement of the vaccination schedule in the countries where rabies is endemic is essential. In addition to the type of vaccine, injection routes also contribute to enhanced immune responses and increased potency of the vaccines. The vaccines approved by the World Health Organization (WHO) include cell culture and embryonated egg-based rabies vaccines (CCEEVs). In order to develop a vaccine against rabies, it is necessary to use an appropriate delivery system to promote a proper antigen-specific immune response.&nbsp;Different routes of injection such as intradermal&nbsp;(ID),&nbsp;intramuscular&nbsp;(IM) or subcutaneous (SC) are practiced, with controversies over their suitability. In this article,&nbsp;we discuss the immunological aspects of rabies vaccination by comparing ID and IM delivery systems. Rabies Vaccine, Rabies, Immunization Schedule, Vaccination, Lyssavirus 77 86 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-172-1&slc_lang=en&sid=1 Mahsa Golahdooz mahsa.ko73@yahoo.com 0000-0002-9575-6382 No Department of Virology, Pasteur Institute of Iran, Tehran, Iran Sana Eybpoosh 0000-0002-2304-8352 No Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran Rouzbeh Bashar dr.r.bashar@gmail.com 0000-0001-5469-4472 No Department of Virology, Pasteur Institute of Iran, Tehran, Iran Mahsa Taherizadeh taherizadeh.mahsa@yahoo.com 0000-0002-9632-037X No Department of Virology, Pasteur Institute of Iran, Tehran, Iran Behzad Pourhossein pourhossein.b@gmail.com 0000-0002-4263-0612 No Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohamadreza Shirzadi shirzadim@gmail.com 0000-0002-7993-8362 No Zoonosis Control Department, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran Behzad Amiri 0000-0002-0952-4957 No Department of Zoonosis Control, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran Maryam Fazeli m_fazeli@pasteur.ac.ir 0000-0003-4048-4778 Yes Department of Virology, Pasteur Institute of Iran, Tehran, Iran