Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1396
10
1
gregorian
2018
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1
6
1
online
1
fulltext
en
Distribution of Genes Encoding Iron Uptake Systems among the Escherichia coli Isolates from Diarrheal Patients of Iran
Microbial pathogenesis
Microbial pathogenesis
Original article
Original article
<strong>Introduction: </strong>Diarrheagenic <em>Escherichia</em> <em>coli</em> (DEC) including enteropathogenic (EPEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and shiga toxin producing <em>E. coli</em> are among the most common agents of diarrhea. There are various classes of iron uptake receptors, but there is not much data on the presence of these iron receptors in DEC isolates. The present study aimed to evaluate the presence of iron receptor genes and also hemolysis activity in these isolates. <strong>Methods</strong>: Totally, 88 DEC isolates (EAEC, ETEC, STEC, and EPEC) from a previous microbial collection were included in this study. The isolates were tested for the production of hemolysin on blood agar plates. Then, Polymerase Chain Reaction (PCR) was used for detection of iron acquisition genes, including <em>chuA</em>, <em>hma</em>, <em>iroN</em>, <em>fyuA</em>, <em>iutA</em> and <em>ireA</em>. <strong>Results</strong>: Our results showed that 8 (66.7%), 25 (89.3%), 17 (44.4%) and 10 (83.4%) of EPEC, STEC, ETEC and EAEC isolates, respectively had hemolytic activity. All the EPEC isolates were negative for <em>hma</em> gene, and <em>iroN</em> and <em>ireA</em> genes were absent in the EAEC isolates. The frequency of <em>chuA</em>, <em>hma</em> and <em>fyuA</em> genes in the STEC and EAEC isolates was higher, whereas EPEC and ETEC isolates revealed a higher frequency of <em>iroN</em> gene than the STEC and EAEC isolates. <strong>Conclusion</strong>: This study reports the presence of various iron receptor genes with a significant hemolysin activity in DEC isolates from Iran. The presence of these genes may contribute to the increased pathogenesis of these isolates in the intestinal tract.
Diarrheagenic, Escherichia coli, Iron receptors, Hemolysin, PCR
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http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-164-2&slc_lang=en&sid=1
Aida
Hajihosein-Tabrizi
m_habibi@pasteur.ac.ir