Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1396
4
1
gregorian
2017
7
1
5
3
online
1
fulltext
en
Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<strong>Introduction:</strong> The strains of<em> Pseudomonas aeruginosa</em> are known as an opportunistic pathogen that can cause infections in humans and animals. Metallo-β-lactamases (MBLs) are the most significant factors of resistance to carbapenem antibiotics in these bacteria. This study was designed to identify the MBLs producing <em>P. aeruginosa</em> isolates in three hospitals of Tehran, Iran. <strong>Methods: </strong>Totally, we obtained 665 samples from patients hospitalized in three hospitals in Tehran, Iran. Antibiotic-susceptibility test of the <em>P. aeruginosa</em> isolates was done based on Kirby-Bauer disk diffusion test. The Minimum Inhibitory Concentration (MIC) of the isolates was performed using agar dilution method, and IPM-EDTA test identified MBL producing isolates. <strong>Results:</strong> Among the examined isolates, 473 (71.1%) were <em>P. aeruginosa</em>. Among these, 306 (64.7%) were resistant to imipenem, and 289 (94.5%) were MBL producers. Furthermore, the resistance rate of the isolates to other antibiotics was amikacin (26%), tobramycin (24.95%), ceftazidime (23.05%), gentamicin (22.83%), carbenicillin (21.14%), and ceftizoxime (18.19%). The MICs of imipenem and ceftazidime for the majority of the isolates were 4 µg/ml and ˃128 µg/ml, respectively. <strong>Conclusion: </strong>This study confirmed previous reports on the increased rate of MBL-mediated resistance in <em>P. aeruginosa</em> isolates worldwide. Therefore, detection of resistance patterns for these isolates, particularly MBLs, is necessary for prevention and control of <em>Pseudomonas</em> associated infections.
Pseudomonas aeruginosa, Metallo-β-lactamase, IPM-EDTA, MIC
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50
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-164-1&slc_lang=en&sid=1
Mohsen
Tabasi
mohsentabasi68@gmail.com