Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1401
6
1
gregorian
2022
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1
10
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online
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fulltext
en
SARS-CoV-2 Variants Screening in Burkina Faso
Diagnostic/screening methods and protocols
Diagnostic/screening methods and protocols
Original article
Original article
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Introduction</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">: In Sub-Saharan Africa, the data on the mutations and variants of circulating SARS-CoV-2 is limited. This study aimed to screen specific mutations and variants of SARS-CoV-2 circulating in Burkina Faso. <b>Methods</b>: This study included symptomatic and asymptomatic individuals who underwent diagnostic testing for SARS-CoV-2 by RT-PCR on nasopharyngeal and oropharyngeal swabs from 7 December 2021 to 12 January 2022. Samples from individuals with a Ct value ≤ 33 were selected for the variants-specific mutation screening. The screening was performed using two kits, “SNPsig</span></span><sup><span lang="FR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">®</span></span></sup><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> SARS-COV-2 (Escape PLEX)" and "SNPsig® VariPLEX™ (COVID-19) Real-Time PCR Assay". <b>Results</b>: SARS-CoV-2 prevalence was 18.9% (332/1758). A total of 113 samples (34.04%) had a Ct value less than (≤ 33), with only 20.35% (23/113) belonging to symptomatic patients. The mean age was 39.01±13 years. The Beta variant (B.1.351) was the most detected one comprising 78.8% (89/113) of variants. Gamma and Delta variants were detected at a low proportion of 0.9% (1/113). No mutation or variant was detected in seven (6.2%) samples. <b>Conclusion</b>: Specific mutation screening detected Beta (B.1.351), Gamma (P.1), and Delta (B.1.617.2) variants of SARS-CoV-2 circulating in Burkina Faso. The absence of mutations in some samples might suggest variants other than those detected. </span></span></span></span></span><br>
SARS-CoV-2, RT-PCR, Ct, Variant, Mutation, Burkina Faso
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http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-429-1&slc_lang=en&sid=1
Serge Theophile
Soubeiga
theo.soubeiga@gmail.com