en
jalali
1393
4
1
gregorian
2014
7
1
2
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online
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fulltext
en
A Survey of Medically Important Snails of Gahar Lake in Lorestan Province, Iran
Introduction: Some snails play an important role in the transmission of helminthes, mainly trematodes of medical and veterinary importance. There seems to be no information on the freshwater snails of Gahar Lake in Lorestan province of Iran. The present study aimed to identify medically important snails of this lake. Methods: Samples were collected from ten localities around Gahar Lake in April 2015 by hand. The snails were classified according to shells morphology. The data were then analyzed using descriptive statistics. Results: A total of 6 snail species were collected from all localities. Four medically important snail species including: Lymnaea truncatula, Lymnaea peregra, Melanoides tuberculata and Melanopsis spp. were detected. M. tuberculata was seen in all sampling sites. Physa acuta and Melanopsis spp. were observed in five sampling sites. Planorbis intermixtus, L. peregra and L. truncatula were found in four, three and two sampling sites, respectively. Conclusion: Presence of medically important snails in Gahar Lake could be a source of trematode infections for visitors. Therefore control measures, especially biological ones should be applied to the lake.
Snails, Lakes, Iran, Trematoda
91
94
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-60-1&slc_lang=en&sid=1
2015/07/10
1394/4/19
2015/09/5
1394/6/14
Rouhollah
Valipour Nouroozi
Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
mvn1365@yahoo.com
003194753284600831
003194753284600831
Yes
en
Detection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
Introduction: Toxigenic Clostridium difficile is the major cause of antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is related to toxins A&B. Children with cancer are at risk of developing C. difficile infection (CDI) due to increased exposure to antibiotics, immunosuppression, and longer hospital stays. Recently, due to higher sensitivity and specificity of nucleic acid amplification test (NAATs) compared to toxin enzyme immunoassays (EIAs), many laboratories are transitioning to NAATs for detection of CDI. We aimed to use a multiplex PCR to detect the C. difficile toxin genes tcdA, tcdB and tpi in stool of cancerous children. We also aimed to show the effects of chemotherapy regimens on the prevalence of C. difficile in these children. Methods: 105 fecal samples were collected from cancerous children who were hospitalized and undergoing chemotherapy. The presence of tcdA, tcdB, and tpi genes were tested. Results: C. difficile was identified in 17.14% of children and the detection rate of A-B+ strains was higher than A+B+ and A+B- strains. C. difficile was found in 17.8% of children who received single antibiotic (5/28 cases virulence genes were detected in 4 cases) and in 41.4% of patients who received more than one antibiotics (12/29 cases virulence genes were detected in 8 cases﴿. Conclusion: Multiplex PCR is a powerful technique for preliminary screening and rapid detection of C. difficile and its virulence genes in the stool of cancerous children. The prevalence of C. difficile in cases receiving several antibiotics was higher than those receiving single antibiotics.
Antibiotic Prophylaxis, Cancer, Toxigenic, Clostridium difficile, Multiplex PCR
95
99
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-51-1&slc_lang=en&sid=1
2015/07/102015/05/27
1394/3/6
2015/09/52015/09/6
1394/6/15
Hadis
Tavafi
Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
hadistavafi@yahoo.com
003194753284600832
003194753284600832
No
Parviz
Owlia
Molecular Microbiology Research Center, Shahed University, Tehran, Iran
owlia@yahoo.com
003194753284600833
003194753284600833
No
Fariba
Shirvani
Pediatric Infections Research Center, Mofid Children Hospital, Tehran, Iran
shirvanifariba@rocketmail.com
003194753284600834
003194753284600834
No
Mozhgan
Hashemie
Pediatric Infections Research Center, Mofid Children Hospital, Tehran, Iran
Hashemie@yahoo.com
003194753284600835
003194753284600835
No
Nader
Shahrokhi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
shahrokhi@pasteur.ac.ir
003194753284600836
003194753284600836
Yes
en
Isolation of PVL/ACME-Positive, Community Acquired, Methicillin-Resistant Staphylococcus aureus (USA300) from Iran
Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital- and community-acquired infections. USA300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. Methods: Totally 171 isolates of S. aureus were collected from different clinical samples in selected university hospitals in Mashhad, Tehran, and Isfahan cities. Then, they were assessed by agar screening and disk diffusion methods to determine their resistance to Methicillin. The isolated MRSA strains were confirmed by detection of mecA gene. The staphylococcal cassette chromosome mec (SCCmec), agr, and spa typing and also detection of Panton-Valentine leukocidin (PVL) and arginine catabolic mobile element (ACME) genes were performed on mecA harboring isolates. Multilocus sequence typing was performed on PVL/ACME positive MRSA strains. Results: We found a PVL/ACME positive MRSA isolate. Genetic evaluation results for this isolate produced the following profile: positive for mecA, pvl, arcA, and hla genes, negative for vanA, sec, and tst1, and belonged to agr I, SCCmec IV, sequence type 8 (ST8), and spa t008. Conclusion: Our results suggest a finding of USA300CA-MRSA isolate in Mashhad, Iran. This is an uncommon finding, because USA300 is routinely found in areas other than Middle East. A notable point about these isolates is that they belong to American Endemic clones.
Staphylococcus aureus, Methicillin-Resistant, Panton-Valentine leukocidin, Iran.
100
104
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-62-1&slc_lang=en&sid=1
2015/07/102015/05/272015/08/11
1394/5/20
2015/09/52015/09/62015/09/28
1394/7/6
Amir
Azimian
Department of Pathobiology and Laboratory Science, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
amir_azimian2003@yahoo.com
003194753284600837
003194753284600837
No
Seyed Asghar
Havaei
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
havaei.seyedasghar@gmail.com
003194753284600838
003194753284600838
Yes
kiarsh
Ghazvini
Department of microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
kiarash_ghazvini@yahoo.com
003194753284600839
003194753284600839
No
Mahsa
Khosrojerdi
Department of Pediatrics, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
khosrojerdi_mahsa@yahoo.com
003194753284600840
003194753284600840
No
Mahmood
Naderi
Molecular Biology and Genetic Engineering Department, Stem Cell Technology Research Center, Tehran, Iran.
mahmood.naderi@gmail.com
003194753284600841
003194753284600841
No
Siamak
Samiee
Reference Health Laboratories, Ministry of Health and Medical Education, Tehran, Iran.
siamak_samiee@yahoo.com
003194753284600842
003194753284600842
No
en
Antibiotic Resistance Pattern and Frequency of mecA Gene in Staphylococcus aureus Isolated from Shohada Hospital, Tabriz
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) can cause serious and life-threatening hospital- and community-acquired infections. Colonized and infected patients represent the most important reservoir of MRSA in health care facilities. Therefore, in this study, MRSA isolates collected from Shohada Hospital in Tabriz were evaluated for the frequency of mecA gene and their antimicrobial susceptibility in a period of three years, from 2010 to 2012. Methods: A total of 182 S. aureus isolates were collected from clinical specimens and first genotypically identified by detection of nuc gene. Antimicrobial susceptibility test was performed by disc agar diffusion method using cefazolin, methicillin, tetracycline, and cefoxitin according to clinical and laboratory standards institute (CLSI) recommendation. Phenotypic (cefoxitin 30 µg/disc) and genotypic (mecA gene detection by PCR) methods were used for detecting methicillin sensitivity. Results: All isolates expressed S. aureus specific sequence gene (nuc) in their PCR products. Eighty-one (44.5%) isolates were confirmed as MRSA by cefoxitin disc screening test and 97 (53.3%) isolates by showing the presence of mecA gene. All the methicillin sensitive S. aureus (MSSA) isolates and 64 (66%) MRSA isolates were found to be susceptible to cefazolin, but 25 (25.8%) MRSA were resistant to tetracycline and cefazolin. Conclusion: The results of this study showed high frequency (53.3%) of MRSA with no significant differences in rates within the three years of study, indicating the inefficiency of control programs to care for patients with MRSA.
mecA, Methicillin-Resistant Staphylococcus aureus, Polymerase Chain Reaction, Iran
105
108
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-54-1&slc_lang=en&sid=1
2015/07/102015/05/272015/08/112015/06/15
1394/3/25
2015/09/52015/09/62015/09/282015/09/28
1394/7/6
Farzaneh
Khoei
Department of Microbiology, College of Medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran
farzaneh.khoei@gmail.com
003194753284600843
003194753284600843
No
Haedeh
Mobaiyen
Department of Microbiology, College of Medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran
drhmobaiyen@iaut.ac.ir
003194753284600844
003194753284600844
Yes
Mohamamad Reza
Nahaei
Department of Microbiology, College of Medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran
nahaeim@yahoo.com
003194753284600845
003194753284600845
No
Sanam
Sadeghi Mohammadi
Department of Microbiology, College of Medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran
sanam_sadeghii@yahoo.com
003194753284600846
003194753284600846
No
en
Amino acid Substitution Mutations Analysis of gyrA and parC Genes in Clonal Lineage of Klebsiella pneumoniae conferring High-Level Quinolone Resistance
Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. The objective of this study was to determine the amino acid substitutions of GyrA and ParC proteins in certain clonal lineages of the K. pneumoniae conferring high level quinolone resistance. Methods: One hundred and eleven isolates of K. pneumoniae were recovered from clinical specimens in a teaching hospital in Kerman, Iran. The antibiotic susceptibility and MIC of quinolones were determined according to CLSI guidelines. Clonal lineages of the isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR amplification using ERIC specific primer sequences. Amino acid mutation profile of gyrA and parC amplicons of six high quinolone resistant isolates was also investigated by DNA sequencing. Results: Twenty two isolates were resistant to nalidixic acid (MIC 256µg/ml), ciprofloxacin (MIC 32µg/ml), levofloxacin (MIC 32µg/ml), and ofloxacin (MIC 32 µg/ml). Typing by ERIC-PCR identified 4 clusters and six singleton, the largest one was belonged to cluster-3 obtained from urine samples. Sequencing of gyrA gene showed three amino acid substitutions (Ser83→Ile Lys154→Arg Ser171→Ala) in the strains 18, 20, 33, two mutations (Lysine154→Arg Ser171→Ala) in the strains 27, 65 and six substitutions in the strain 66, of which, three (Ser83→Phe Asp87→Ala Val190→Gly) were unique for this strain. Sequencing of parC gene revealed double substitutions (Ser129→Ala Ala141→Val) in the strains 18, 27, 66 and three aminoacid changes (Ser80→Ile Ser129→Ala Ala141→Val) in the strains 20 and 33 respectively. Alignment and phylogenetic tree analysis of the gyrA sequence from highly quinolone resistant isolate 66 with homologs sequences obtained from the NCBI database confirmed 99.8% similarity to gyrA gene of the K. pneumoniae ha10 (GenBank: JX123017.1). Conclusion: The results of present study suggest that acquisition of mutations in certain positions of gyrA and parC genes confer high level resistance to quinolones.
Klebsiella pneumoniae, quinolones resistance, ERIC-PCR, sequencing, GyrA and ParC mutations.
109
117
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-69-1&slc_lang=en&sid=1
2015/07/102015/05/272015/08/112015/06/152015/09/26
1394/7/4
2015/09/52015/09/62015/09/282015/09/282015/10/19
1394/7/27
Amin
Norouzi
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.
aminnorouzi1988@gmail.com
0031947532846001701
0031947532846001701
No
Omid
Azizi
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.
omid201262@gmail.com
0031947532846001702
0031947532846001702
No
Hossein
Hosseini
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.
hosseini.nave@gmail.com
0031947532846001703
0031947532846001703
No
Samane
Shakibaie
student research committee, kerman university of medical sciences
samaneshakibaie@yahoo.com
0031947532846001704
0031947532846001704
No
Mohammad reza
Shakibaie
Research center for infectious diseases and tropical medicine
mohammadreza.shakibaie@gmail.com
0031947532846001705
0031947532846001705
Yes
en
Detection of Borrelia DNA in Ornithodoros tholozani ticks and their eggs
Introduction: Spirochetes of Borrelia can be visualized directly in infected ticks by dark-field microscopy. Inoculation of in phosphate buffered saline (PBS) suspension of ground Argasid soft ticks to susceptible animals or allowing the ticks to feed on the same species followed by microscopic examination of the animals’ blood have also been practiced. With the advent of molecular methods and introduction of various gene markers, Borrelia persica DNA was detected in Ornithodoros tholozani ticks by using several gene markers, but the data on transovarial transmission of Borrelia in this tick by these methods is very scarce. Methods: In this study we tried to detect Borrelia in field collected O. tholozani ticks by allowing them to feed on guinea pigs and then to examine the animals’ blood for spirochetes by microscopy. We also used two PCR methods targeting highly repetitive regions of rrs gene to detect Borrelia DNA in adult ticks, larvae, and eggs. Results: All the guinea pig blood samples were negative for spirochetes by microscopy. However, out of the 17 adult ticks, 2 males and 5 females were positive for Borrelia DNA. None of the larvae was positive, but two batches of eggs yielded the expected 540 bp amplicon by nested PCR. Conclusion: Presence of Borrelia DNA in adult O. tholozani ticks and their eggs is an indication for transovarial transmission of relapsing fever agent in this tick.
Borrelia persica, Ornithodoros tholozani, Transovarial transmission, PCR, Iran
118
120
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-26-1&slc_lang=en&sid=1
2015/07/102015/05/272015/08/112015/06/152015/09/262015/12/1
1394/9/10
2015/09/52015/09/62015/09/282015/09/282015/10/192015/12/28
1394/10/7
Afsaneh
Aghaei
Islamic Azad University of Lahijan, Microbiology Department, Lahijan, Iran
aghaei_afsoon@yahoo.com
003194753284600852
003194753284600852
No
Behnaz
Ghazinezhad
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
behnaz894@yahoo.com
003194753284600853
003194753284600853
No
Saied Reza
Naddaf
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
snaddaf_2001@yahoo.com
003194753284600854
003194753284600854
Yes
en
Employing PCR Technique in Assessment of Monoclonality in Large B-cell Non-Hodgkin\'s Lymphoma
Introduction: Most B-cell malignancies are diagnosed based on morphologic and immunohistochemical criteria. Some cases, however, still present a challenge for the pathologist to discriminate between reactive hyperplasia and neoplastic disorders. Molecular techniques can be used as a helpful diagnostic tool in these cases. In this study, we assessed the value of polymerase chain reaction (PCR) technique in determination of monoclonality of immunoglobulin heavy chain gene rearrangements for the diagnosis of large B-cell non-Hodgkin;#39s lymphoma (NHL) in paraffin embedded tissue samples. Methods: DNA was extracted from paraffin embedded tissues of 44 diffuse large B-cell lymphoma (DLBCL) cases and 20 samples of reactive lymphoid tissues from appendix and tonsils as control. Framework 3 and the joining region (FR3/JH) of the variable segment of the immunoglobulin heavy chain gene were amplified using degenerated primers. PCR products from each sample were analyzed on 8% polyacrylamide gels following AgNO3 staining. Results: Monoclonal rearrangements were identified in 33 of 44 cases (75%) of DLBCL using FR3/JH primers. Monoclonal IgH gene rearrangements were not detected in any of the reactive lymphoid hyperplasic samples. All these control cases showed polyclonal pattern. Conclusion: Through PCR analysis, using degenerated primers, monoclonality was demonstrated in 75% of DLBCL cases. This technique can thus be used as a sensitive, reliable and valuable diagnostic supplement to conventional morphologic examination and immunohistocytochemical evaluation of lymphoproliferative disorders, particularly in cases with restrictions in amount or type of analytic material.
Immunoglobulin Gene, PCR, Non-Hodgkins Lymphoma
121
124
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-48-2&slc_lang=en&sid=1
2015/07/102015/05/272015/08/112015/06/152015/09/262015/12/12015/06/23
1394/4/2
2015/09/52015/09/62015/09/282015/09/282015/10/192015/12/282015/10/10
1394/7/18
Noushin
Lotfi
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences
noushin_297@yahoo.com
003194753284600855
003194753284600855
No
Maryam
Rastin
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences
rastinm@mums.ac.ir
003194753284600856
003194753284600856
No
Parisa
Shoaei
Nososcomial infection research center, Isfahan University of Medical Sciences,
shoaei@idrc.mui.ac.ir
003194753284600857
003194753284600857
No
Bahram
Memar
Department of Pathology, School of Medicine, Mashhad University of Medical Sciences,
noushin_297@yahoo.com
003194753284600858
003194753284600858
No
Nafiseh Sadat
Tabasi
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences
shin_297@yahoo.com
003194753284600859
003194753284600859
No
Zohreh
Mahmoudi
School of Medicine, Mashhad University of Medical Sciences
shin_297@yahoo.com
003194753284600860
003194753284600860
No
Reza
Alimohammadi
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
shin_297@yahoo.com
003194753284600861
003194753284600861
No
Behnam
Yousefi
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
shin_297@yahoo.com
003194753284600862
003194753284600862
No
Mahmoud
Mahmoudi
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences
mahmoudim@mums.ac.ir
003194753284600863
003194753284600863
Yes
en
Species Identification of Acanthamoeba Strains Isolated from Patients Referring to Farabi Eye Reference Center Using PCR-RFLP Method
Introduction: Acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. Infection with Acanthamoeba spp. poses threat to human health, such as, Acanthamoeba keratitis (AK) that is a vision-threatening infection of the cornea. This study aimed to identify the species of Acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Methods: Acanthamoeba isolates investigated in this cross-sectional study, were collected from patients referring to Farabi Eye Reference Center. All 10 isolates were subjected to species identification using DNA based method. BspLI (NlaIV) and HpyCH4IV restriction enzymes were used to categorize the PCR amplified DNA by PCR-RFLP method. Results: Six samples were identified as Acanthamoeba palestinensis and 4 isolates as Acanthamoeba culbertsoni, which implies that all the isolates belong to pathogenic strains of Acanthamoeba. Conclusion: Acanthamoeba can enter the corneal tissue and survive in the eye, which results in AK. To the authors' knowledge, no study is available on species identification of this genus using these enzymes and technique. This is the first time in Iran that Acanthamoeba isolates are subjected to species identification using PCR-RFLP method.
Acanthamoeba Keratitis, Cornea, DNA Restriction Enzymes, PCR-RFLP
125
129
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-68-1&slc_lang=en&sid=1
2015/07/102015/05/272015/08/112015/06/152015/09/262015/12/12015/06/232015/09/23
1394/7/1
2015/09/52015/09/62015/09/282015/09/282015/10/192015/12/282015/10/102015/09/29
1394/7/7
Mir Mostafa
Ghamilouie
Pasteur Institute of Iran, Tehran
m.ghamilooye@yahoo.com
003194753284600960
003194753284600960
No
Zarrintaj
Valadkhani
Pasteur Institute of Iran, Tehran
valad.zarrin@gmail.com
003194753284600961
003194753284600961
Yes
Fariba
Khoshzaban
003194753284600962
003194753284600962
No