en
jalali
1396
4
1
gregorian
2017
7
1
5
3
online
1
fulltext
en
Detection of Brucella spp. in the Sheep and Goats Milks from Southeastern Iran Using Culture and PCR
Introduction: Brucellosis is a zoonotic disease caused by the members of the genus Brucella. This bacterium is transmitted to humans through exposure to infected animals or via consumption of contaminated dairy products. Cultivation of the bacteria or amplification of its DNA using polymerase chain reaction (PCR) are conventional diagnostic approaches for definitive identification of Brucella in raw milk. Method: We collected 530 milk samples from 485 healthy animals, and 45 animals with a history of abortion from Kerman province, southeast Iran. The specimens were first cultured in Eugon broth and then were subcultured on Brucella agar. Gram smears from colonies for characterization of the bacteria were prepared. Also, DNA extraction and PCR amplification of IS711 fragment were performed to detect Brucella DNA in the milk samples. Results: The culture method detected Brucella Spp. in 10 milk samples including two samples from apparently healthy animals (1 sheep sample, and 1 goat sample) as well as eight samples from animals with abortion history (6 sheep samples, and 2 goat samples). PCR identified Brucella DNA in 43 samples including those from healthy sheep (n=4) and goats (n=9), as well as animals milk with abortion history (7 sheep, and 23 goats). The proportion of positive samples detected by PCR method was significantly higher than culture method (P=0.014). Conclusion: The PCR assay turned to be a convenient method for detection of Brucella contamination of raw milk and can be used as a reliable tool for surveillance and screening of contaminated milk.
Brucella, PCR, Culture, Goat, Sheep.
40
42
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-155-1&slc_lang=en&sid=1
2017/12/31
1396/10/10
2018/02/3
1396/11/14
Zahra
Shirazi
Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran
dr.shirazi.mail@gmail.com
0031947532846002140
0031947532846002140
No
Mohammad
Khalili
Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran
mdkhalili1@yahoo.com
0031947532846002141
0031947532846002141
No
Balal
Sadeghi
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
sadeghi.balal@gmail.com
0031947532846002142
0031947532846002142
Yes
Hamid
Sharifi
HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
sharifihami@gmail.com
0031947532846002143
0031947532846002143
No
ShahrnazBanuo
Ashrafganjooyi
Research Center for Tropical and Infectious Disease, Kerman University of Medical Sciences, Kerman, Iran
sharifihami@gmail.com
0031947532846002144
0031947532846002144
No
en
Cysticercus fasciolaris (Taenia taeniaeformis Larval Stage) in Urban Rats with Illustration of Histopathological Changes in the Liver
Introduction: Cysticercus fasciolaris is the larval stage of the cestode Taenia taeniaeformis, whose definitive hosts are cats. Rodents and very rarely humans act as intermediate hosts. Here, we investigated the rate of infection among urban rats in the north of Khuzestan province, southwest of Iran, and described the histopathological changes in the liver of infected animals. Methods: One hundred eight rats were collected from the cities Dezful, Sush, and Andimeshk in the north of Khuzestan province during 2014-2015. The rats were sacrificed, dissected, and their livers were removed and examined macroscopically for the presence of the cyst, which were then cut open and inspected for larvae. The cysts of infected livers were examined for the histopathological changes and the recovered larvae were identified based on morphometric features. Results: Out of 108 rats examined, 8 (7.4%) had small to moderate size cysts. Each cyst had two layers and contained one larva of C. fasciolaris. The scolex of the larvae had four suckers and a rostellum armed with two rows of hooks. The infected livers had multiple cysts and liver parenchyma necrosis or neoplastic evidence were not seen in the tissue sections. Conclusion: The small size cysts indicated actue infection, which may explain the absence of sarcoma as well.
Cysticercus fasciolaris, Rats, Histopathological changes
43
46
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-154-2&slc_lang=en&sid=1
2017/12/312018/02/11
1396/11/22
2018/02/32018/02/26
1396/12/7
Hamid
Hasanpour
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
molaviva@tums.ac.ir
0031947532846001982
0031947532846001982
Yes
Faezeh
Najafi
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
faezeh_najafi@yahoo.com
0031947532846001983
0031947532846001983
No
Mohammad Javad
Gharagozlou
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mjavad@ut.ac.ir
0031947532846001984
0031947532846001984
No
Sanaz
Jafarpour Azami
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
molaviva@tums.ac.ir
0031947532846001985
0031947532846001985
No
Arezoo
Fadavi
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
molaviva@tums.ac.ir
0031947532846001986
0031947532846001986
No
Niloofar
Paknezhad
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
molaviva@tums.ac.ir
0031947532846001987
0031947532846001987
No
Gholamreza
Mowlavi
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
molaviva@tums.ac.ir
0031947532846001988
0031947532846001988
No
en
Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran
Introduction: The strains of Pseudomonas aeruginosa are known as an opportunistic pathogen that can cause infections in humans and animals. Metallo-β-lactamases (MBLs) are the most significant factors of resistance to carbapenem antibiotics in these bacteria. This study was designed to identify the MBLs producing P. aeruginosa isolates in three hospitals of Tehran, Iran. Methods: Totally, we obtained 665 samples from patients hospitalized in three hospitals in Tehran, Iran. Antibiotic-susceptibility test of the P. aeruginosa isolates was done based on Kirby-Bauer disk diffusion test. The Minimum Inhibitory Concentration (MIC) of the isolates was performed using agar dilution method, and IPM-EDTA test identified MBL producing isolates. Results: Among the examined isolates, 473 (71.1%) were P. aeruginosa. Among these, 306 (64.7%) were resistant to imipenem, and 289 (94.5%) were MBL producers. Furthermore, the resistance rate of the isolates to other antibiotics was amikacin (26%), tobramycin (24.95%), ceftazidime (23.05%), gentamicin (22.83%), carbenicillin (21.14%), and ceftizoxime (18.19%). The MICs of imipenem and ceftazidime for the majority of the isolates were 4 µg/ml and ˃128 µg/ml, respectively. Conclusion: This study confirmed previous reports on the increased rate of MBL-mediated resistance in P. aeruginosa isolates worldwide. Therefore, detection of resistance patterns for these isolates, particularly MBLs, is necessary for prevention and control of Pseudomonas associated infections.
Pseudomonas aeruginosa, Metallo-β-lactamase, IPM-EDTA, MIC
47
50
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-164-1&slc_lang=en&sid=1
2017/12/312018/02/112018/03/5
1396/12/14
2018/02/32018/02/262018/03/14
1396/12/23
Mohsen
Tabasi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
mohsentabasi68@gmail.com
0031947532846002154
0031947532846002154
No
Reza
Azizian
Massoud Clinical Laboratory, Tehran, Iran
mohsentabasi68@gmail.com
0031947532846002155
0031947532846002155
No
Mohammad Reza
Eskandarion
Iranian Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran
mohsentabasi68@gmail.com
0031947532846002156
0031947532846002156
No
Mehri
Habibi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
m_habibi1362@yahoo.com
0031947532846002157
0031947532846002157
No
Mohammad Reza
Asadi Karam
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
m_asadi12@yahoo.com
0031947532846002158
0031947532846002158
Yes
en
Molecular Detection of Antibiotic Resistance Determinants in Lactobacillus Bacteria Isolated from Human Dental Plaques
Introduction: Dental caries is a significant public health problem which destroys the calcified tissues of the teeth. The frequency of lactobacilli in dental caries is usually high. The objective of this study was to evaluate the incidence of these bacteria in dental caries and to identify the antibiotic resistance patterns of them. Methods: Specimens from dental plaque were collected from 30 patients. Phenotypic (Gram staining, cell morphology, and biochemical tests) and molecular (PCR) methods were used for identification of Lactobacillus bacteria. The antimicrobial sensitivity test was performed by standard Kirby-Bauer disk diffusion method to determine their resistance to chloramphenicol, penicillin, vancomycin, nitrofurantoin, cefixime, cefazolin, amoxicillin-clavulanic acid, and tetracycline. Antibiotic resistance genes were detected using multiplex PCR method as well. Results: Fourteen out of 30 (46/6%) samples were Lactobacillus positive. The mecA was the essential gene responsible for penicillin resistance. Also, the blaTEM and blaOXA-1 genes had more significant role in cefazolin and cefixime resistance than blaSHV, and these two genes had the similar role in the amoxicillin-clavulanic acid resistance. Also, tetracycline resistance was caused by both tetK and tetM genes identically. Conclusion: In conclusion, Lactobacillus bacteria had an essential role in the formation of dental plaque and showed multidrug-resistant patterns to commonly used antibiotics.
Dental plaque, Lactobacillus, Multiplex PCR, resistance genes
51
55
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-116-2&slc_lang=en&sid=1
2017/12/312018/02/112018/03/52017/10/24
1396/8/2
2018/02/32018/02/262018/03/142018/02/10
1396/11/21
Sahar
Nouri Gharajalar
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
saharnouri@yahoo.com
0031947532846001994
0031947532846001994
Yes
Masoumeh
Firouzamandi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
0031947532846001995
0031947532846001995
No
en
The Role of the Gray Wolf in Rabies Transmission in Iran and Preliminary Assessment of an Oral Rabies Vaccine in this Animal
Introduction: In Iran, 95% of animal bites are attributed to domestic dogs and cats, while one-third of rabies victims are from wildlife. The wolf is one of the primary vectors of wildlife in the country. This study was aimed to assess the impact of wildlife on rabies transmission and to evaluate the efficacy of an oral rabies vaccine (ORV) in the gray wolf (Canis lupus pallipes). Methods: We studied the incidence of wolf attacks by analyzing a 10-year period of data (2000 to 2009) available in the Ministry of Health and Medical Education, the Veterinary Organization and Pasteur Institute of Iran. In the next step, five captive gray wolves were fed by Raboral V-RG® vaccine baits (Merial®, part of Boehringer Ingelheim, Germany) and monitored for rabies neutralizing antibodies over 20 months by rapid fluorescent focus inhibition test (RFFIT). Results: Our analysis showed that 55% of animal bites occurred in rural areas and the remaining in urban areas. The most rabid wolves were from Fars province in southern Iran. The vaccine baits were palatable for the hungry wolves. Seroconversion with antibodies titers ≥0.5 IU/ml occurred after 12 weeks and maintained for 78 weeks indicating potential protection against rabies after receiving a single ORV bait. Conclusion: Our results suggested that a single dose of vaccination with V-RG®, though initially designed for foxes and raccoons, can protect the gray wolf against rabies as well.
Oral rabies vaccination (ORV), Rabies, anti-rabies antibodies, wolf
56
61
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-160-1&slc_lang=en&sid=1
2017/12/312018/02/112018/03/52017/10/242018/02/20
1396/12/1
2018/02/32018/02/262018/03/142018/02/102018/02/24
1396/12/5
Alireza
Gholami
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
a.gholami@pasteur.ac.ir
0031947532846001996
0031947532846001996
Yes
Siamak
Massoudi
Department of Environment, Wildlife Diseases Group, Wildlife Bureau, Tehran, Iran
siamak_massoudi@yahoo.com
0031947532846001997
0031947532846001997
No
Majid
Kharazian Moghaddam
Department of Environment, Iran
mkhmoghaddam@yahoo.com
0031947532846001998
0031947532846001998
No
Mahmoud
Ghazi Marashi
Department of Environment, Wildlife Diseases Group, Wildlife Bureau, Tehran, Iran
mahmoud_marashi_iauk@yahoo.com
0031947532846001999
0031947532846001999
No
Mahyar
Marashi
Department of Environment, Wildlife Diseases Group, Wildlife Bureau, Tehran, Iran
mahyarmarashi@yahoo.com
0031947532846002000
0031947532846002000
No
Rouzbeh
Bashar
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
dr.r.bashar@gmail.com
0031947532846002001
0031947532846002001
No
Ahmad
Fayaz
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
fayaz@pasteur.ac.ir
0031947532846002002
0031947532846002002
No
Maryam
Fazeli
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
fazelim9@gmail.com
0031947532846002003
0031947532846002003
No
Firouzeh
Farahtaj
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
farahtaj@pasteur.ac.ir
0031947532846002004
0031947532846002004
No
Nader
Howaizi
National Reference Center for Rabies, Pasteur Institute of Iran, Tehran, Iran
howaizi362@yahoo.com
0031947532846002005
0031947532846002005
No
Mohammad Reza
Shirzadi
Department of Zoonoses, Center for Communicable Disease Control, Ministry of Health and Medical Education, Tehran, Iran
shirzadim@gmail.com
0031947532846002006
0031947532846002006
No
en
Comparison of Pyogenic, Tuberculous and Brucellar Spondylitis during 10 Years in a Central City of Iran
Introduction: Spondylitis is a serious disease caused by a variety of pathogens. The identification of spondylitis etiologies is a very important medical issue. This study was conducted to compare clinical, laboratory and radiological features of the patients with tuberculous (TS), brucellar (BS) and pyogenic spondylitis (PS) in a central city of Iran. Methods: In this retrospective study, we obtained the data of 100 patients with spondylitis from a hospital in Arak city. The patients were divided into three groups including TS (8 cases), BS (71 cases) and PS (21 cases), based on the spondylitis etiology. Results: The mean age of cases with TS, BS, and PS was 67.25±20.26, 55.39±15.60 and 52.19±12.74 years, respectively. The most common clinical feature was back pain followed by fever. Twenty-one cases had psoas abscess which was more common in PS group. No significant difference regarding the involved vertebral regions was observed between the groups. Intravenous drug use, history of vertebral surgery and chronic renal failure were frequent in patients with PS, and all TS cases had pulmonary involvement. Conclusion: Our data showed that presence of concomitant pulmonary involvement and a confirmed history of tuberculosis are suggestive of tuberculous spondylitis. However, the distinction between TS and BS is still problematic and only a combination of clinical data, laboratory findings, radiological features and history of TB can be helpful in differentiation of TS and BS.
Spondylitis, Brucellosis, Tuberculosis, Pyogenic
62
65
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-165-1&slc_lang=en&sid=1
2017/12/312018/02/112018/03/52017/10/242018/02/202018/04/7
1397/1/18
2018/02/32018/02/262018/03/142018/02/102018/02/242018/04/11
1397/1/22
Masoomeh
Sofian
Infectious Disease Research Center (IDRC), Department of Infectious Disease, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
dr.sofian@arakmu.ac.ir
0031947532846002007
0031947532846002007
No
Arezoo
Aghakhani
Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
araghakhani@hotmail.com
0031947532846002008
0031947532846002008
No
Fatemeh
Pourbaferani
Students Research Committee, Arak University of Medical Sciences, Arak, Iran
dr.fatemeh5160@yahoo.com
0031947532846002009
0031947532846002009
No
Mohammad
Banifazl
Iranian Society for Support of Patients with Infectious Diseases, Tehran, Iran
mohammadbanifazl@aol.com
0031947532846002010
0031947532846002010
No
Nader
Zarinfar
Infectious Disease Research Center (IDRC), Department of Infectious Disease, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
nzarinfar@yahoo.com
0031947532846002011
0031947532846002011
No
Manije
Kahbazi
Infectious Disease Research Center (IDRC), Department of Pediatrics Infectious Disease, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
m_kahbazi77@yahoo.com
0031947532846002012
0031947532846002012
No
Amitis
Ramezani
Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
amitisramezani@hotmail.com
0031947532846002013
0031947532846002013
Yes
en
Detection of a Brucella-like (Alphaproteobacteria) Bacterium in Boophilus spp. (Acari: Ixodidae) from Iran
Ticks harbor many pathogenic, as well as endosymbiotic and non-pathogenic agents. They are host of a variety of as yet unidentified microbes that continue to be described. In the present study, a Brucella-like bacterium was detected in a Boophilus tick by PCR amplification of a partial fragment of 16S rRNA locus followed sequencing. Our results show that the members of the genus Boophilus may act as vectors of brucellosis in nature, but further studies are required to confirm the real role of ticks as vector or reservoirs of specific Brucella species.
16S rRNA, phylogenetic tree, Brucellaceae, Boophilus
66
68
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-151-1&slc_lang=en&sid=1
2017/12/312018/02/112018/03/52017/10/242018/02/202018/04/72017/10/28
1396/8/6
2018/02/32018/02/262018/03/142018/02/102018/02/242018/04/112018/02/10
1396/11/21
Asadollah
Hosseini-Chegeni
Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khorramabad, Iran
hosseinichegeni@gmail.com
0031947532846002132
0031947532846002132
Yes
Majid
Tavakoli
Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran
Faghihi.f@iums.ac.ir
0031947532846002133
0031947532846002133
No
Zakkyeh
Telmadarraiy
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Faghihi.f@iums.ac.ir
0031947532846002134
0031947532846002134
No
Mohammad Mehdi
Sedaghat
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Faghihi.f@iums.ac.ir
0031947532846002135
0031947532846002135
No
Faezeh
Faghihi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Faghihi.f@iums.ac.ir
0031947532846002136
0031947532846002136
No
en
Reply: Surveillance of Crimean-Congo Haemorrhagic Fever in Pakistan
[No Abstract]
To the editor: In a recent publication entitle “Surveillance of Crimean-Congo haemorrhagic fever in Pakistan” published in “The Lancet Infectious Diseases”, the authors claimed that before the Eid al-Adha, Crimean-Congo Hemorrhagic Fever (CCHF)-livestock infected are imported from Iran and Afghanistan to Baluchistan province of Pakistan and then transferred to other areas of the country
Crimean-Congo Haemorrhagic Fever, Pakistan
69
70
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-157-1&slc_lang=en&sid=1
2017/12/312018/02/112018/03/52017/10/242018/02/202018/04/72017/10/282018/02/5
1396/11/16
2018/02/32018/02/262018/03/142018/02/102018/02/242018/04/112018/02/102018/02/7
1396/11/18
Ehsan
Mostafavi
Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging infectious diseases, Pasteur Institute of Iran, Tehran, Iran
Mostafaviehsan@gmail.com
0031947532846002137
0031947532846002137
Yes
Kourosh
Holakouie-Naieni
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Science, Tehran, Iran
holakoik@hotmail.com
0031947532846002138
0031947532846002138
No
Mostafa
Salehi Vaziri
Department of Arboviruses and Viral Hemorrhagic Fevers, Research Centre for Emerging and Reemerging infectious diseases, Pasteur Institute of Iran, Tehran, Iran
Mostafavaziri1985@gmail.com
0031947532846002139
0031947532846002139
No