en Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article <p dir="ltr"><strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;">Introduction:</span></span></strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;"> Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of <em>icaABCD</em> genes in clinical isolates of MRSA. <strong>Methods:</strong> A total of 110 clinical <em>Staphylococcus aureus</em> isolates were collected from two teaching hospitals in Shahrekord (Hajar and Kashani hospitals). The MRSA isolates were detected by an antibiotic susceptibility test. A microtiter tissue plate assay was used to detect the phenotypic biofilm formation. A polymerase chain reaction (PCR) was performed to detect the presence of <em>icaABCD</em> genes. <strong>Results:</strong> The microtiter plate assay results showed that attachment abilities were strong in 26 (23.6%) strains, moderate in 30 (27.2%) strains, and weak in 16 (14.54%) strains. The prevalence of the <em>icaA</em>, <em>icaB</em>, <em>icaC</em>, and <em>icaD</em> genes among the studied isolates were as follows: 42 isolates were <em>icaA</em> positive (38.18%), 34 <em>icaB</em> positive (30.9%), 46 <em>icaC</em> positive (41.8%), and 50 were <em>icaD</em> positive (45.4%). <strong>Conclusion:</strong> The high prevalence of <em>icaA/D</em> harboring <em>S. aureus</em> among the clinical isolates suggests that the risk of persistent infections in the hospital settings is considerably high.</span></span></p> Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, icaABCD 67 70 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-91-1&slc_lang=en&sid=1 Safiyeh Abbasi safiyehabbasi@rocketmail.com Yes Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran Behnam Zamanzad safiyehabbasi@rocketmail.com No Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran