Journal of Medical Microbiology and Infectious Diseases

en Detection of Different Types of Metallo-β-Lactamases among Pseudomonas aeruginosa Isolates Obtained from Intensive Care Unit Patients Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article Introduction:<span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-bidi-font-weight: bold mso-ansi-language: EN-US mso-fareast-language: KO"> <a name="OLE_LINK13">Pseudomonas aeruginosa</a> is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to <a name="OLE_LINK9"></a><a name="OLE_LINK8">various antimicrobial agents, </a>such as <a name="OLE_LINK10">Metallo-β-</a>Lactams (MBL). This study aimed to investigate the prevalence <a name="OLE_LINK26"></a><a name="OLE_LINK21">of β-lactamase</a>s encoding genes among P. aeruginosa strains isolated from intensive care unit (ICU) patients by phenotypic and multiplex PCR methods. Methods:<span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-bidi-font-weight: bold mso-ansi-language: EN-US mso-fareast-language: KO"> A total of 48 non-duplicate strains of P. aeruginosa were collected from different clinical specimens of patients hospitalized in ICU wards of a teaching hospital in Isfahan, Iran. Susceptibility test was performed by disk diffusion method. All meropenem resistant strains were subjected to modified Hodge test (MHT) for detection of carbapenemases. Multiplex PCRs were performed to detect β-lactam-resistant P. aeruginosa isolates. Results:<span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-bidi-font-weight: bold mso-ansi-language: EN-US mso-fareast-language: KO"> In disk diffusion method, P. aeruginosa strains showed the most (97.9%) resistance against imipenem and meropenem and the least (45.8%) against colistin. Thirty-six (75%) out of the 48 isolates were multidrug resistant. PCR amplification of β-lactamase genes showed the presence of blaVIM genes in 7 (14.6%) and <a name="OLE_LINK30"></a><a name="OLE_LINK29">blaIMP </a>in 15 (31.3%) strains. Also, blaSME, SPM, GIM, AIM and NDM genes were not observed in any of the strains. We only found a statistically significance difference between the presence of blaIMP gene and multidrug-resistant (MDR) positivity and source of specimen (p=0.009 and 0.002, respectively). Conclusion:<span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: Arial mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-bidi-font-weight: bold mso-ansi-language: EN-US mso-fareast-language: KO"> Rapid and reliable identification of MBLs appears to be necessary for effective treatment of related infections. <a name="OLE_LINK17"></a><a name="OLE_LINK16">Besides, our results may provide useful perception to make a more appropriate choice of antibiotics, which may put a stop to carbapenem-resistant infections.</a> Pseudomonas aeruginosa, PCR, Carbapenem, Beta-lactamase 84 90 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-48-1&slc_lang=en&sid=1 Farzin Khorvash khorvash@med.mui.ac.ir No Department of Infectious Diseases, Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Mohammad reza Yazdani yazdani@med.mui.ac.ir Yes Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Shiva Shabani dr.shiva_shabani90@yahoo No Department of Infectious Diseases, Isfahan University of Medical Sciences, Isfahan, Iran Houri Alizadeh houri.alizadeh@yahoo.com No Department of Microbiology, Science and Research, Islamic Azad University, Shiraz, Iran Ali asghar Soudi A_soudi_2005@yahoo.com No Department of Infectious Diseases, Isfahan University of Medical Sciences, Isfahan, Iran Parisa Shoaei shoaei@idrc.mui.ac.ir No Department of Microbiology, Science and Research, Islamic Azad University, Shiraz, Iran Behrooz Ataei ataei@med.mui.ac.ir No Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Majid Yaran yaranmajid@yahoo.com No Department of Infectious Diseases, Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran