Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1393
1
1
gregorian
2014
4
1
2
2
online
1
fulltext
en
Assessment of Alkaline Phosphatase Activity in Hydatid Cyst Protoscolices and Liver Tissue as a Pathological Biomarker
Animal Models
Animal Models
Original article
Original article
<p><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO"><strong>Introduction: </strong></span><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO mso-bidi-font-weight: bold">Hydatid cyst disease is caused by the protoscolices of <i>Echinococcus granulosus</i>. Alkaline phosphatase (ALP) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. The aim of this study was to compare the level of ALP activity (as a pathological biomarker) in hydatid cyst protoscolices (HCP) with that of sheep liver tissue and to determine the effect of cystic infection on the enzyme activity. </span><b style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">Methods:</span></b><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO mso-bidi-font-weight: bold"> HCPs were collected from sheep livers with hydatid cysts at a local abattoir and washed 3 times with PBS buffer. HCP samples were freeze-thawed and sonicated, while the collected liver tissues were homogenized. Then, extract solutions were centrifuged and stored at -20°C. ALP activity was measured in the extract solutions of HCP and healthy and infected sheep liver tissue samples. The amounts and bands of protein samples were detected using Bradford method and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively. To determine the significant difference between the two groups, independent two samples t-test was used. </span><b style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">Results:</span></b><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO mso-bidi-font-weight: bold"> The mean values of ALP-specific activity of healthy and infected livers and HCP were estimated 0.019, 0.175, and 1.28 U/ml/mg, respectively. Higher ALP activity level was observed in cystic liver compared to healthy liver (p<0.05). T-test analysis showed higher ALP enzyme activity for HCP compared to healthy liver (p<0.05). SDS-PAGE demonstrated a protein band with molecular weight of 59 kDa in HCP samples, which was identified as ALP. </span><b style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">Conclusion:</span></b><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: 'Times New Roman' mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO mso-bidi-font-weight: bold"> ALP activity in HCP and healthy liver indicates the importance of this enzyme in comparative biochemistry of liver and parasite. Higher level of ALP enzyme activity in cystic liver in comparison with healthy liver could be considered as a pathological biomarker for diagnosis of hydatid cyst disease with other hydatid disease parameters</span><span style="FONT-FAMILY: "Garamond",serif FONT-SIZE: 10pt mso-bidi-font-family: Arial mso-fareast-font-family: Batang mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO mso-bidi-font-weight: bold">.</span></p>
Alkaline phosphatase, Hydatid cyst, Liver, Parasite
68
70
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-47-1&slc_lang=en&sid=1
Mojgan
Farrokhi Karibozorg
1003194753284600626
1003194753284600626
No
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Ali
Farahnak
farahnak@tums.ac.ir
1003194753284600627
1003194753284600627
Yes
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Bagher
Molaei Rad
1003194753284600628
1003194753284600628
No
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Taghi
Golmohammadi
1003194753284600629
1003194753284600629
No
Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Reza
Eshraghian
1003194753284600630
1003194753284600630
No
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran