Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1401
6
1
gregorian
2022
9
1
10
3
online
1
fulltext
en
Prevalence of Constitutive and Inducible Clindamycin Resistance among Methicillin-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kashmir Valley
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<b><span lang="EN-IN" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Introduction: </span></span></span></b><span lang="EN-IN" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">The increased frequency of Methicillin<i>-</i>resistant <i>Staphylococcus aureus</i> infections has led to renewed interest in the macrolide-lincosamide streptogramin B (MLS) group of antibiotics. Resistance to these antibiotics may be constitutive or inducible. Isolates resistant to erythromycin may show false <i>in vitro</i> susceptibility to clindamycin, leading to therapeutic failures. This study investigated the utility of the D-Test for detecting inducible clindamycin resistance in methicillin-resistant <i>S. aureus</i> isolates and determining the prevalence of various phenotypes in our region<span style="color:#c00000">.</span> <b>Methods: </b>For detecting inducible clindamycin resistance, a D-test using erythromycin and clindamycin as per CLSI guidelines was performed, and four different phenotypes were interpreted as methicillin-sensitive (MS) phenotype (D-test negative), inducible MLSB (iMLSB) phenotype (D-test positive), constitutive MLSB phenotype and sensitive to both. <b>Results</b>: Of the 987 isolates tested, 400 (40.53%) were MRSA. The prevalence of iMLSB, cMLSB phenotype, MS phenotype and sensitive phenotype in MRSA isolates was 42.5%, 10.5%, 28% and 19%, respectively. The iMLSB and cMLSB phenotypes were higher in males (24.75%, 6.25%) than females (<i>P</i>-value = 0.137). The majority of MRSA isolates originated from pus (83%). All <i>S. aureus</i> isolates showed 100% sensitivity to vancomycin and linezolid. <b> Conclusion</b>: This study emphasizes the prevalence of inducible clindamycin resistance in MRSA in our setup. Incorporating the D-test into the routine Kirby–Bauer disk diffusion method in clinical microbiology laboratories will help clinicians make judicious use of clindamycin, minimizing treatment failure.</span></span></span>
MRSA, D-test, Constitutive clindamycin resistance, Inducible clindamycin resistance
104
113
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-396-1&slc_lang=en&sid=1
Shahida
Akhter
karnainovais@gmail.com
10031947532846005369
10031947532846005369
Yes
Department of Microbiology, Government Medical College, Srinagar, India
Asifa
Nazir
asifanazir@gmail.com
10031947532846005370
10031947532846005370
No
Department of Microbiology, Government Medical College, Srinagar, India
Ovais
Karnain
drovaiskarnain@gmail.com
10031947532846005371
10031947532846005371
No
Department of Microbiology, Government Medical College, Srinagar, India
Mariya
Rouf
drmariyaovais@gmail.com
10031947532846005372
10031947532846005372
No
Department of Microbiology, Government Medical College, Srinagar, India