Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1401
6
1
gregorian
2022
9
1
10
3
online
1
fulltext
en
Molecular Characterizations and Antimicrobial Susceptibility of Extended-Spectrum ß-lactamase (ESBL) Producing Proteus spp. Clinical Isolates in Babol, Northern Iran
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0e101a">Introduction: </span></span></span></span></b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0e101a">Proteus </span></span></span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0e101a">spp. are opportunistic members of <i>Enterobacteriaceae</i>, accounting for 10% of urinary tract infections and other primary clinical infections. They produce extended-spectrum beta-lactamases (ESBL) that can confer resistance to beta-lactam antibiotics. This study aimed t</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">o investigate the prevalence, antimicrobial susceptibility, molecular characteristics, and genetic relationship of ESBL-producing <i>Proteus</i> spp. clinical isolates in Babol, Northern Iran.<b><span style="color:#0e101a"> Methods:</span></b><span style="color:#0e101a"> In this cross-sectional study, out of 112 clinical samples, 30 <i>Proteus </i>spp<i>.</i> isolates were identified via specific biochemical assays. </span>According to the Clinical and Laboratory Standards Institute (CLSI) guidelines, antibiotic susceptibility was evaluated using disc diffusion and agar dilution methods, and polymerase chain reaction (PCR) was used to detect <i>bla</i><sub>TEM</sub><span style="color:#0e101a"> and <i>bla</i><sub>SHV</sub> </span>genes. <b><span style="color:#0e101a">Results:</span></b> The resistance rate to tetracycline and sulfamethoxazole was highest <span style="color:black">by </span>disk diffusion and agar dilution. Multiple drug-resistant (MDR) isolates were 86% and 60% in disk diffusion and agar dilution assays. Seven (23.3%) isolates had the <i>bla</i><sub>TEM</sub> genes and 18 (60%) <i>bla</i><sub>SHV</sub>. <b>Conclusion:</b> ESBL-producing <i>Proteus </i>spp. was highly prevalent, and the <i>bla</i><sub>SHV</sub> was the most common resistance contributing gene. These findings and relatively high resistance to ampicillin demand more care in prescribing antibiotics. Also, the high prevalence of MDR isolates in patients infected with ESBL-producing <i>Proteus</i> spp. requires continuous surveillance. </span></span></span>
Proteus spp, Beta-lactamase, Multi-drug resistance, Antibiotic resistance
114
121
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-228-4&slc_lang=en&sid=1
Atena
Malaki
amir9226012@gmail.com