Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1400
12
1
gregorian
2022
3
1
10
1
online
1
fulltext
en
Detection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Short communication
Short communication
<strong>Introduction:</strong> In the past decade, multidrug-resistant <em>Acinetobacter baumannii</em> has become one of the most critical challenges in treating infected patients. The AdeABC efflux pump is the most important among the various resistance mechanisms. This pump can force various antibiotics and ethidium bromide out of the bacterial cell to the surrounding environment. <strong>Methods:</strong> In this study, nine <em>A. baumannii</em> clinical isolates were isolated and identified using different biochemicals (catalase, oxidase, TSI, hemolysis, growth at 44°C, and indole) and molecular (<em>bla</em><sub>Oxa51</sub> gene) tests. Following the antibiogram test, the antibiotic resistance changes in the isolates in the presence and absence of efflux pump inhibitor (CCCP) were determined for tetracycline, and ciprofloxacin AdeABC efflux pump genes, including <em>adeA</em>, <em>adeB</em>, and <em>adeC,</em> were amplified by PCR. Finally, the presence of the AdeABC efflux pump was investigated using the agar ethidium-bromide cartwheel method (AEBCM). <strong>Results: </strong>According to the antibiogram test, all isolates were MDR. In the presence of efflux pump inhibitor, a reduced resistance for tetracycline was observed, but not for ciprofloxacin. The AdeABC efflux pump genes were detected in all isolates. An increase in the AdeABC pump activity in four isolates was confirmed using AEBCM.<strong> Conclusion:</strong> AEBCM, a fast and convenient tool for assessing the ethidium bromide secretion in various bacteria, provides a quick diagnosis and treatment of multidrug-resistant bacteria.
Agar ethidium bromide cartwheel, AdeABC, Acinetobacter baumannii
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41
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-374-1&slc_lang=en&sid=1
Amin
Sepehr
aminsepehr20@gmail.com
10031947532846004986
10031947532846004986
No
Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran
Sepideh
Fereshteh
fereshteh.sepideh@yahoo.com
10031947532846004987
10031947532846004987
No
Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran.
Nader
Shahrokhi
nader.shahrokhi@gmail.com
10031947532846004988
10031947532846004988
Yes
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran