Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1400
9
1
gregorian
2021
12
1
9
4
online
1
fulltext
en
Identification of Carbapenemase within Class 1 Integron Structure in Intrinsically Colistin-resistant Enterobacteriaceae
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<strong>Introduction:</strong> Identification of carbapenem resistance genes in intrinsically colistin-resistant Enterobacteriaceae (ICRE), i.e., <em>Proteus</em> spp., <em>Providencia</em> spp., <em>Serratia</em> spp., and <em>Morganella</em> spp., is necessary for preventing such life-threatening bacteria. Here, we characterized <a name="_Hlk84557444">carbapenemase resistance genes and their</a> carriage within class 1 integron structures in clinical intrinsically colistin-resistant Enterobacteriaceae. <strong>Methods:</strong> Clinical samples were collected from six general hospitals in Tehran and Torbat-e-Heydarieh, Iran, from 2018 to 2020. The isolates were identified by standard microbiological techniques and 16s rRNA sequencing. The resistance to imipenem and colistin was determined by disk diffusion and minimum inhibitory concentration (MIC) assays. PCR-sequencing was performed to detect primary carbapenemase-encoding genes, including <em>bla</em><sub>OXA-48</sub>, <em>bla</em><sub>IMP</sub>, <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>KPC,</sub> and <em>bla</em><sub>NDM</sub>. <strong>Results:</strong> Three <em>Proteus mirabilis</em>, three<em> Providencia stuartii, </em>and two <em>Serratia marcescens</em> isolates were identified. The MIC<sub>IMP</sub> values of all isolates were >16 µg/ml. The <em>bla</em><sub>VIM-1 </sub>gene and <em>bla</em><sub>KPC-2</sub> genes were detected in five and four isolates, respectively. Cassette arrays consisting of <em>bla</em><sub>VIM-1</sub>-<em>aacA7</em>-<em>dhfrA1</em>-<em>aadA1</em>were detected in five isolates and <em>aadB</em> in one isolate. The <em>bla</em><sub>VIM-1 </sub>gene in all <em>bla</em><sub>VIM-1 </sub>positive isolates was located on class 1 integrons, while the <em>bla</em><sub>KPC-2 </sub>carriage was not related to these genetic elements. The <em>in silico</em> analysis of the large plasmids carrying<em> bla</em><sub>VIM-1</sub>-<em>aacA7</em>-<em>dhfrA1</em>-<em>aadA1</em> array belonging to the IncA/C incompatibility group showed transmissibility among Enterobacteriaceae members.<strong> Conclusions:</strong> Transferable plasmid harboring <em>bla</em><sub>VIM-1 </sub>in ICREs can pose a severe threat to public health. More investigations are required to depict the epidemiology of these underestimated carbapenem-resistant Enterobacteriaceae members.
Antimicrobial resistance, Carbapenemase, Integron, Plasmid
203
209
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-150-2&slc_lang=en&sid=1
Negin
Bolourchi
neginbolourchi@gmail.com