Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1401
9
1
gregorian
2022
12
1
10
4
online
1
fulltext
en
The adeH and adeS Efflux Pump Genes in Imipenem and Colistin-Resistant Acinetobacter baumannii Clinical Isolates
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Introduction: </span></span></span></b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Acinetobacter baumannii</span></span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> is one of the most important causes of nosocomial infections. In this bacteria, several mechanisms contribute to resistance against antimicrobial agents. The present study investigated the prevalence of <i>adeS</i> and <i>adeH</i> genes and the role of efflux pumps in imipenem and colistin-resistant <i>A. baumannii </i>clinical isolates<i>.</i> <b>Methods: </b>This study included 60 <i>A. baumannii</i> isolates collected from medical centers affiliated with the Shahid Beheshti University of Medical Science, Tehran, Iran. The antibiotic susceptibility pattern was examined using the broth microdilution MIC method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Also, the <i>adeS</i> and <i>adeH</i> genes were amplified by PCR.<b> Results: </b>The isolates were 100% imipenem-resistant and 86.7% colistin-resistant. All isolates were positive for the <i>51-blaOXA</i> gene. The <i>adeH</i> and <i>adeS</i> genes were detected in 95% and 80% of the isolates. <b>Conclusion: </b>The high frequency of <i>adeS </i>and<i> adeH</i> efflux pump genes and the high drug resistance in <i>A. baumannii </i>clinical isolates indicated that <i>adeS </i>and<i> adeH</i> efflux pump genes contribute to antibiotic resistance in this species. Therefore, our results provide essential information about high drug resistance in <i>A. baumannii </i>clinical isolates that can help limit the horizontal and vertical transmission of efflux pump genes in antibiotic-resistant <i>A. baumannii</i> isolates that causes nosocomial infections in susceptible strains.</span></span></span>
Acinetobacter baumannii, Efflux pump, adeS gene, adeH gene
186
191
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-366-1&slc_lang=en&sid=1
Azam
Aghili Amjad
Aghiliamjad.a@gmail.com