Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1392
8
1
gregorian
2013
11
1
1
1
online
1
fulltext
en
Role of imiquimod as adjuvant for vaccination against Leishmania major infection in BALB/c mice
Original article
Original article
<p><span font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><strong>Various adjuvants in combination with different antigens have been utilized as a vaccine candidate against leishmaniasis. However, the search for ideal adjuvants is still pursued due to the inefficiencies of current compounds. In the present study, the effect of imiquimod, as an adjuvant, is studied with soluble <i>Leishmania</i> antigens (SLA) in BALB/c mice. Four groups of mice were immunized with SLA, SLA plus imiquimod, SLA plus BCG, and PBS as control. Immunized mice received a boosting dose of SLA after 15 days. All groups were challenged with <i>Leishmania major (L. major) </i>promastigotes 2 weeks after the booster immunization. Our results showed that strong TH1 responses were induced in groups of SLA plus imiquimod as well as SLA plus BCGafter immunization. These responses included smaller footpad thickness, lower parasite load in lymph node, and higher proliferative response of lymph node cells to SLA, higher levels of interferon &gamma in culture supernatant of lymph node cells, and higher levels of IgG, and IgG2a in sera. The data supports the possibility of using imiquimod as a suitable adjuvant in <i>leishmania</i> vaccination. </strong></span></p>
Imiquimod, Leishmania major, BCG, Adjuvant, Vaccination
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35
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-25-10&slc_lang=en&sid=1
Faramarz
Dobakhti
1003194753284600671
1003194753284600671
No
School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Ghader
Khalili
1003194753284600672
1003194753284600672
Yes
Immunology Department, Pasteur Institute of Iran, Tehran, Iran
Hamid
Mahmoudzadeh Niknam
1003194753284600673
1003194753284600673
No
Immunology Department, Pasteur Institute of Iran, Tehran, Iran
Vahid
Khaze
1003194753284600674
1003194753284600674
No
Immunology Department, Pasteur Institute of Iran, Tehran, Iran
Fatemeh
Partovi
1003194753284600675
1003194753284600675
No
Faculty of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Taraneh
Naghibi Mahmoodabadi
1003194753284600676
1003194753284600676
No
School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Shahriar
Aalinejad
1003194753284600677
1003194753284600677
No
Kasra Hospital, Tehran, Iran