Journal of Medical Microbiology and Infectious Diseases Journal of Medical Microbiology and Infectious Diseases JoMMID Medical Sciences http://jommid.pasteur.ac.ir 1 admin 2345-5349 2345-5330 8 10.61882/JoMMID 14 8888 13 en jalali 1400 6 1 gregorian 2021 9 1 9 3 online 1 fulltext
en Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article <strong>Introduction:</strong> The rapidly increasing extended-spectrum &beta;-lactamase-producing <em>Pseudomonas aeruginosa</em> is a threat to health. This study aims to detect the <em>rpoD</em> gene and <em>bla<sub>OXA-10</sub></em> and <em>bla<sub>OXA-48</sub></em> genes in imipenem-resistant <em>P. aeruginosa</em> clinical isolates simultaneously by multiplex polymerase chain reaction. <strong>Methods:</strong> Eighty-five culture plates were collected from patients suspected of <em>Pseudomonas</em> spp infection in Ghaem Hospital and Shahid Shourideh Clinic in Mashhad from January to February 2021. After biochemical identification of <em>P. aeruginosa</em> isolates and the measurement of antibiotic resistance, <em>bla<sub>OXA-10</sub></em>, <em>bla<sub>OXA-48</sub></em>, and <em>rpoD</em> genes were investigated by multiplex polymerase chain reaction in the imipenem-resistant isolates. <strong>Results:</strong> Of 82 <em>P. aeruginosa</em> isolates, 38 (46.34%) were resistant to imipenem, with the highest percentage to carbenicillin (69.5%). All imipenem-resistant <em>P. aeruginosa</em> isolates were confirmed by multiplex PCR using the primers that targeted the <em>rpoD</em> gene. Also, in multiplex PCR, among imipenem-resistant isolates, 10 (26.3%) and 9 (23.6%) had<em> bla<sub>OXA-10</sub></em> and <em>bla<sub>OXA-48</sub></em> genes, respectively. <strong>Conclusion:</strong> In addition to molecular identification of <em>P. aeruginosa</em>, the present study simultaneously detected <em>bla<sub>OXA-10</sub></em> and <em>bla<sub>OXA-48</sub></em> genes by multiplex PCR. Application of this multiplex PCR, rapid identification of patients, and timely treatment can reduce the &beta;-lactamase gene prevalence in <em>P. aeruginosa</em> clinical isolates. Pseudomonas aeruginosa, Multiplex PCR, rpoD gene, blaOXA-10 gene, blaOXA-48 gene 142 147 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-333-1&slc_lang=en&sid=1 Aghdas Bibi Hashemi hashemiag1@gmail.com 0000-0002-1745-1364 No Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mahboobeh Nakhaei Moghaddam mahboobe_nak@yahoo.com 0000-0002-3567-5951 No Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mohammad Mahdi Forghanifard forghanifard@damghaniau.ac.ir 0000-0002-7691-6897 No Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University, Damghan, Iran Ehsan Yousefi ehyousefi@yahoo.com 0000-0003-4873-2983 Yes Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran