Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1400
3
1
gregorian
2021
6
1
9
2
online
1
fulltext
en
Frequency of cbrA, cbrB, ndvB, and phoBR Genes in Relation to Biofilm Formation in Pseudomonas aeruginosa Clinical Isolates
Microbial pathogenesis
Microbial pathogenesis
Original article
Original article
<strong>Introduction: </strong>After <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em> is the third cause of hospital-acquired infection (HAI). This bacteria's ability to colonize in different environments, especially in hospitals and biofilm formation, has added to its impact as an HAI. The molecular mechanism of biofilm formation is not well understood, but several genes contribute to this phenomenon. This study investigates the frequency of <em>cbrA</em>, <em>cbrB</em>, <em>phoBR, </em>and<em> ndvB</em> genes in biofilm-forming <em>P. aeruginosa</em> isolates.<strong> Methods: </strong>Fifty <em>P. aeruginosa</em> clinical isolates were collected from various sources such as urine, ulcer, blood, secretions, and trachea in Milad Hospital, Tehran, from 2017 to 2018. Biofilm formation in the isolates was assessed by the microtiter plate assay, and the frequency of <em>cbrA</em>, <em>cbrB</em>, <em>phoBR</em>, and <em>ndvB</em> genes was investigated by PCR.<strong> Results: </strong>Among the 50 isolates, 44% were strong biofilm former, 34% moderate biofilm former, 12% weak biofilm former, and 10% did not form biofilms. PCR revealed a frequency of 94% for the <em>cbrA</em> gene, 78% for <em>cbrB</em>, 96% for <em>ndvB</em>, and 48% for <em>phoBR</em>. The coexistence of all four genes was 68% in strong biofilm former isolates, 41% in moderate biofilm former isolates, 37% in weak biofilm former, and zero in the isolates that formed no biofilm.<strong> Conclusion:</strong> The high frequency of <em>ndvB</em> and <em>cbrA</em> genes and the coexistence of <em>ndvB</em> and <em>cbrB</em> suggest the contribution of these genes in the biofilm formation of <em>P. aeruginosa. </em>
Pseudomonas aeruginosa, Biofilm, cbrA, cbrB, phoBR, ndvB
97
102
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-290-2&slc_lang=en&sid=1
Mahla
Tajmir Riahy
Mahla@gmail.com
10031947532846004454
10031947532846004454
No
Department of Microbiology, Islamic Azad University, Varamin- Pishva Branch, Varamin, Iran
Sahar
Honarmand Jahromi
sahar.honarmand@yahoo.com
10031947532846004455
10031947532846004455
No
Department of Microbiology, Islamic Azad University, Varamin- Pishva Branch, Varamin, Iran.
Mansoor
Khaledi
mansoor.khaledi@yahoo.com
10031947532846004456
10031947532846004456
No
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
Hamed
Afkhami
Afkhami@gmail.com
10031947532846004457
10031947532846004457
No
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
Maryam
Shafaati
maryam.shafaati@gmail.com
10031947532846004458
10031947532846004458
No
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Hamid
Lava Khamseh
Hamaidh@gmail.com
10031947532846004459
10031947532846004459
No
Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
Shohreh
Zare Karizi
h.a.university.ac@gmail.com
10031947532846004460
10031947532846004460
Yes
Department of Genetic, Islamic Azad University, Varamin- Pishva Branch, Varamin, Iran.