en Epidemiologic studies including microbial genotyping, phenotyping and serotyping Epidemiologic studies including microbial genotyping, phenotyping and serotyping Original article Original article <strong>Introduction: </strong>Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis with a considerable fatality rate. The virus is mainly transmitted to humans and animals through the bites of infected hard ticks belonging to the genus <em>Hyalomma</em>. This study aimed to determine the prevalence of CCHFV in ticks in Shahr-e Ray city, one of the meat supplier counties in Tehran Province, Iran.<strong> Methods: </strong>A molecular survey was carried out on collected ticks from sheep in slaughterhouses and husbandries of Shahr-e Ray city, during 2016-17. The ticks were identified using taxonomic characteristics, and the presence of CCHF virus RNA was investigated using an RT-PCR amplifying a 536 bp of the small segment of the viral genome.<strong> Results:</strong> The results of this study showed that the <em>Hyalomma</em> ticks were the dominant species in Shahr-e Ray slaughterhouses and husbandries. In none of the assayed ticks, RT-PCR detected CCHFV RNA.<strong> Conclusion:</strong> A considerable tick infestation with members of genus <em>Hyalomma</em>, among sheep in Shahr-e Ray slaughterhouses and husbandries, indicates the vulnerability of this county of tick-borne infections outbreaks such as CCHF. Therefore, applying acaricides and other preventive strategies should be adopted to control the tick population in Shahr-e Ray. Crimean-Congo Hemor-rhagic Fever Virus, Tick, Iran 56 59 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-246-1&slc_lang=en&sid=1 Pedram Talaie pedram.talaie@yahoo.com 0000-0001-7815-7540 No Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran Mohammad Mehdi Sedaghat sedaghmm@tums.ac.ir 0000-0002-1365-0796 No Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ehsan Mostafavi mostafaviehsan@gmail.com 0000-0002-1997-517X No Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran Zakiyeh Telmadarraiy 0000-0002-6975-9571 No Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mehdi Rouhani Kia.rohani@gmail.com 0000-0001-8956-5072 No Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran Mostafa Salehi-Vaziri mostafavaziri1985.2@gmail.com 0000000188928505 Yes Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran.