Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1398
10
1
gregorian
2020
1
1
8
1
online
1
fulltext
en
The Effects of Ellagic Acid on Growth and Biofilm Formation of Candida albicans
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<strong>Introduction: </strong>Biofilm formation is one of the specific features of <em>Candida albicans</em> that protects it from antifungal agents and the host immune system. Also, Biofilm formation by <em>C. albicans</em> on the mucosal surfaces and medical devices are responsible for causing <em>Candida</em> nosocomial infection. Here, we investigated the effects of ellagic acid on <em>C. albicans</em> growth and biofilm formation regarding the expression of two essential genes that are involved in adhesion and yeast-hypha transition. <strong>Methods:</strong> The yeasts were treated with serial two-fold concentrations of ellagic acid (3.125-100 µg/ml) for 48 h at 35°C. The weights of the cultured yeasts were measured as an indicator of the fungal growth, and the biofilm formation was assessed by a tetrazolium salt (XTT) reduction assay. The expressions of <em>HWP1</em> and <em>ALS3</em> genes were assayed by real-time PCR. <strong>Results:</strong> Ellagic acid inhibited <em>C. albicans</em> growth 0.68%-82.44%, dose-dependently. The biofilm formation also reduced 2.61%-68.318%. Also, The expressions of <em>HWP1</em> and <em>ALS3</em> genes were notably suppressed by ellagic acid at different concentrations. <strong>Conclusion:</strong> Our results showed that ellagic acid is a potential candidate to eliminate <em>C. albicans</em>-generated biofilm by suppressing the expression of the involved genes.
Ellagic acid, Candida albicans, Biofilm, Gene
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http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-131-1&slc_lang=en&sid=1
Sepideh
Nejatbakhsh
baharnb181991@yahoo.com
10031947532846003222
10031947532846003222
No
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran
Mohsen
Ilkhanizadeh-Qomi
mohsenilkhani3@gmail.com
10031947532846003223
10031947532846003223
No
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran
Mehdi
Razzaghi-Abyaneh
10031947532846003224
10031947532846003224
No
Department of Mycology, Pasteur Institute of Iran, Tehran
Zahra
Jahanshiri
zjahanshiri@gmail.com
10031947532846003225
0000000194319335
Yes
Department of mycology, Pasteur Institute of Iran, Tehran, Iran